Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RNZ43_RS02150 Genome accession   NZ_CP135143
Coordinates   375272..375709 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain L33a     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 370272..380709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNZ43_RS02115 - 370871..370999 (-) 129 Protein_377 hypothetical protein -
  RNZ43_RS02120 - 371144..372346 (+) 1203 WP_070081342.1 GTP-binding protein -
  RNZ43_RS02125 - 372411..373547 (+) 1137 WP_416406046.1 zinc-dependent alcohol dehydrogenase -
  RNZ43_RS02130 - 373562..373996 (+) 435 WP_021494203.1 RDD family protein -
  RNZ43_RS02135 - 374114..374389 (+) 276 WP_369719109.1 YckD family protein -
  RNZ43_RS02140 - 374603..374833 (+) 231 Protein_382 family 1 glycosylhydrolase -
  RNZ43_RS02145 nin/comJ 374852..375250 (-) 399 WP_077721770.1 competence protein ComJ Regulator
  RNZ43_RS02150 nucA/comI 375272..375709 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RNZ43_RS02155 hxlB 376070..376627 (-) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  RNZ43_RS02160 hxlA 376624..377259 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  RNZ43_RS02165 - 377491..377853 (+) 363 WP_016936795.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=885561 RNZ43_RS02150 WP_003156588.1 375272..375709(-) (nucA/comI) [Bacillus velezensis strain L33a]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=885561 RNZ43_RS02150 WP_003156588.1 375272..375709(-) (nucA/comI) [Bacillus velezensis strain L33a]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641