Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OCUAc18_RS02535 Genome accession   NZ_AP024802
Coordinates   535827..536627 (+) Length   266 a.a.
NCBI ID   WP_001984465.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain OCU Ac18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530827..541627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCUAc18_RS02510 (OCUAc18_04800) - 531737..533035 (+) 1299 WP_086231294.1 glycosyltransferase family 1 protein -
  OCUAc18_RS02515 (OCUAc18_04810) - 533060..533611 (+) 552 WP_000669564.1 phosphatase PAP2 family protein -
  OCUAc18_RS02520 (OCUAc18_04820) iscX 533711..533911 (+) 201 WP_000524330.1 Fe-S cluster assembly protein IscX -
  OCUAc18_RS02525 (OCUAc18_04830) ndk 534025..534456 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  OCUAc18_RS02530 (OCUAc18_04840) rlmN 534579..535811 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OCUAc18_RS02535 (OCUAc18_04850) pilF 535827..536627 (+) 801 WP_001984465.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OCUAc18_RS02540 (OCUAc18_04860) - 536618..537409 (+) 792 WP_000405161.1 RodZ domain-containing protein -
  OCUAc18_RS02545 (OCUAc18_04870) ispG 537431..538546 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OCUAc18_RS02550 (OCUAc18_04880) hisS 538543..539835 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  OCUAc18_RS02555 (OCUAc18_04890) - 539864..540562 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30084.40 Da        Isoelectric Point: 9.2307

>NTDB_id=88547 OCUAc18_RS02535 WP_001984465.1 535827..536627(+) (pilF) [Acinetobacter baumannii strain OCU Ac18]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=88547 OCUAc18_RS02535 WP_001984465.1 535827..536627(+) (pilF) [Acinetobacter baumannii strain OCU Ac18]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment