Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OSC47_RS17295 Genome accession   NZ_CP151161
Coordinates   3581389..3582822 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 20220828076     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3576389..3587822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSC47_RS17260 (OSC47_17270) elbB 3577081..3577734 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  OSC47_RS17265 (OSC47_17275) mtgA 3577731..3578459 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  OSC47_RS17270 (OSC47_17280) yrbL 3578456..3579088 (-) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  OSC47_RS17275 (OSC47_17285) npr 3579301..3579573 (-) 273 WP_266351886.1 PTS phosphocarrier protein NPr -
  OSC47_RS17280 (OSC47_17290) rapZ 3579570..3580424 (-) 855 WP_000243741.1 RNase adapter RapZ -
  OSC47_RS17285 (OSC47_17295) ptsN 3580470..3580961 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OSC47_RS17290 (OSC47_17300) hpf 3581079..3581366 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  OSC47_RS17295 (OSC47_17305) rpoN/rpoN1 3581389..3582822 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  OSC47_RS17300 (OSC47_17310) lptB 3582870..3583595 (-) 726 WP_266351885.1 LPS export ABC transporter ATP-binding protein -
  OSC47_RS17305 (OSC47_17315) lptA 3583602..3584159 (-) 558 WP_266351884.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  OSC47_RS17310 (OSC47_17320) lptC 3584128..3584703 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  OSC47_RS17315 (OSC47_17325) kdsC 3584700..3585266 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  OSC47_RS17320 (OSC47_17330) kdsD 3585287..3586273 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  OSC47_RS17325 (OSC47_17335) yrbG 3586287..3587264 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=884635 OSC47_RS17295 WP_000809051.1 3581389..3582822(-) (rpoN/rpoN1) [Escherichia coli strain 20220828076]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=884635 OSC47_RS17295 WP_000809051.1 3581389..3582822(-) (rpoN/rpoN1) [Escherichia coli strain 20220828076]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465