Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AADH43_RS02220 Genome accession   NZ_CP151155
Coordinates   472851..474317 (-) Length   488 a.a.
NCBI ID   WP_031855957.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain vp-pir-201806     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467851..479317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADH43_RS02190 (AADH43_02190) pmbA 467939..469282 (+) 1344 WP_005459755.1 metalloprotease PmbA -
  AADH43_RS02195 (AADH43_02195) mgtE 469359..470714 (-) 1356 WP_005468659.1 magnesium transporter -
  AADH43_RS02200 (AADH43_02200) - 470934..471209 (-) 276 WP_005468661.1 HPr family phosphocarrier protein -
  AADH43_RS02205 (AADH43_02205) rapZ 471212..472075 (-) 864 WP_005468663.1 RNase adapter RapZ -
  AADH43_RS02210 (AADH43_02210) ptsN 472090..472536 (-) 447 WP_005467453.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AADH43_RS02215 (AADH43_02215) hpf 472539..472826 (-) 288 WP_005467451.1 ribosome hibernation promoting factor -
  AADH43_RS02220 (AADH43_02220) rpoN/rpoN1 472851..474317 (-) 1467 WP_031855957.1 RNA polymerase factor sigma-54 Machinery gene
  AADH43_RS02225 (AADH43_02225) lptB 474368..475093 (-) 726 WP_005467801.1 LPS export ABC transporter ATP-binding protein -
  AADH43_RS02230 (AADH43_02230) lptA 475095..475589 (-) 495 WP_005467803.1 lipopolysaccharide transport periplasmic protein LptA -
  AADH43_RS02235 (AADH43_02235) lptC 475570..476133 (-) 564 WP_025504095.1 LPS export ABC transporter periplasmic protein LptC -
  AADH43_RS02240 (AADH43_02240) kdsD 476224..477195 (-) 972 WP_020841311.1 arabinose-5-phosphate isomerase KdsD -
  AADH43_RS02245 (AADH43_02245) - 477232..478197 (-) 966 WP_031855958.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54826.61 Da        Isoelectric Point: 4.3441

>NTDB_id=884501 AADH43_RS02220 WP_031855957.1 472851..474317(-) (rpoN/rpoN1) [Vibrio parahaemolyticus strain vp-pir-201806]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLEVEEGHEEPQANGEDKSALETADNSANEPTETE
VPDSSDVIEKSEISSELEIDTTWDDVYSANTGSTGLALDDDMPVYQGETTESLHDYLMWQLDLTPFSETDRTIAIAIIDA
IDDYGYLTLSPEEIHESFDNEDIELDEVEAVRKRIQQFDPLGVASRNLQECLLLQLATFPEDTPWLAEAKMILADHIDHL
GNRDYKLVIKETKLKEADLREVLKLIQQLDPRPGSRITPDDTEYVIPDVSVFKDHGKWTVSINPDSIPKLKVNQQYAQLG
KGNSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDVALAVDMHESTISRVTTQKF
MHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAP
SSQRKRLL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=884501 AADH43_RS02220 WP_031855957.1 472851..474317(-) (rpoN/rpoN1) [Vibrio parahaemolyticus strain vp-pir-201806]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCACAGTTGCAACAAGCGATTCGCTTGCTGCA
GTTGTCGACTCTCGATCTTCAACAAGAAATCCAAGAAGCACTGGACTCAAACCCGCTATTGGAAGTTGAAGAAGGCCACG
AAGAACCTCAAGCAAATGGTGAAGACAAATCAGCACTCGAAACTGCTGACAACAGTGCCAACGAGCCAACAGAAACCGAA
GTTCCTGATAGTTCTGACGTCATCGAAAAGTCCGAAATCAGCTCAGAGCTTGAAATCGACACAACTTGGGATGACGTCTA
CAGCGCAAATACGGGCAGTACCGGTTTAGCGTTAGACGATGATATGCCGGTCTACCAAGGCGAAACCACAGAATCGCTGC
ACGATTACTTAATGTGGCAACTCGACTTAACGCCTTTCAGTGAAACCGACCGTACGATTGCCATTGCCATCATTGATGCG
ATTGACGATTACGGCTATCTGACCCTATCTCCTGAAGAGATTCACGAAAGCTTCGACAACGAAGATATTGAGTTGGATGA
GGTCGAAGCCGTGCGCAAACGCATCCAACAGTTTGACCCGCTTGGTGTGGCATCGCGGAACCTGCAAGAATGCTTACTAC
TGCAACTTGCAACCTTCCCAGAAGACACGCCTTGGCTTGCGGAAGCCAAAATGATTCTTGCCGATCACATCGATCACCTT
GGTAATCGCGATTACAAACTGGTCATCAAAGAAACTAAGCTAAAAGAAGCGGATCTACGCGAAGTATTAAAACTGATCCA
ACAGTTAGATCCTCGTCCGGGCAGCCGCATTACCCCAGACGACACAGAATACGTCATTCCGGACGTATCGGTATTTAAAG
ATCATGGGAAATGGACCGTTTCCATCAACCCTGACAGCATTCCAAAGTTAAAAGTTAACCAACAATACGCTCAATTAGGC
AAAGGCAACAGTGCGGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCAAAATGGTTAATTAAGAGCTTAGAGAGCAG
AAACGAGACGCTACTCAAAGTTGCAAGATGTATTGTTGAACATCAACAAGATTTCTTCGAGTATGGTGAAGAAGCCATGA
AGCCAATGGTACTCAATGATGTAGCCTTGGCGGTGGACATGCATGAATCCACAATATCTCGTGTTACGACACAAAAGTTC
ATGCATACGCCACGTGGCATTTTTGAATTGAAGTACTTCTTCTCCAGCCACGTCAGTACAGACAATGGCGGAGAATGTTC
GTCAACAGCAATCCGTGCACTCATTAAGAAACTGGTCGCGGCAGAGAATACTGCTAAACCACTTAGTGACAGTAAGATTG
CTGCTCTTCTGGCTGACCAAGGGATTCAGGTCGCAAGACGTACGATAGCTAAATATCGAGAATCCTTGGGTATTGCCCCT
TCAAGTCAGCGTAAACGCCTACTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.444

100

0.459