Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ROL27_RS08235 Genome accession   NZ_CP134883
Coordinates   1763204..1764643 (+) Length   479 a.a.
NCBI ID   WP_010929513.1    Uniprot ID   -
Organism   Bordetella parapertussis strain 400431-b     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1758204..1769643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROL27_RS08220 (ROL27_08220) - 1759031..1760284 (+) 1254 WP_076879839.1 amidase -
  ROL27_RS08225 (ROL27_08225) leuA 1760425..1762131 (-) 1707 WP_010929515.1 2-isopropylmalate synthase -
  ROL27_RS08230 (ROL27_08230) - 1762558..1763205 (-) 648 WP_010929514.1 hypothetical protein -
  ROL27_RS08235 (ROL27_08235) rpoN/rpoN1 1763204..1764643 (+) 1440 WP_010929513.1 RNA polymerase factor sigma-54 Machinery gene
  ROL27_RS08240 (ROL27_08240) - 1764736..1765038 (+) 303 WP_003815905.1 bacterioferritin-associated ferredoxin -
  ROL27_RS08245 (ROL27_08245) - 1765066..1765944 (-) 879 WP_010929512.1 LysR substrate-binding domain-containing protein -
  ROL27_RS08250 (ROL27_08250) - 1766052..1766981 (+) 930 WP_010929511.1 DMT family transporter -
  ROL27_RS08255 (ROL27_08255) bfr 1767320..1767796 (+) 477 WP_003815898.1 bacterioferritin -
  ROL27_RS08260 (ROL27_08260) - 1767878..1768561 (-) 684 WP_010929510.1 hypothetical protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52283.53 Da        Isoelectric Point: 4.8638

>NTDB_id=884334 ROL27_RS08235 WP_010929513.1 1763204..1764643(+) (rpoN/rpoN1) [Bordetella parapertussis strain 400431-b]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAELSQALAENPLLERDEDPAREEQADPEREASVEADEAP
DTELRLDEMPGSGGVLPDDDSDLPQAARPDTLREHLLGQLALTRAAPRGAALCGLLIDELDENGYLDSPLEEILTWLPAE
AQADLDELRAALALLQSFDPAGIGARDMAECLQLQLRQPDPARLPEAADAEVLACARRICAQHLPLLATGNVARVREAVQ
CDEAVVRAAHALILRLEPRPGRAWTVPAADYAIPDVIVRRTRQGWQAVLNSAVVPRLHVNAVYAQVLGGQRQGGNAGLQA
QLQQARWMIRNVEQRFDTILRVAQAIVAHQQAFFTQGPAAMRPLILKDIAGELDLHESTISRATTQKYMLTPFGTLELKR
FFGAGVSTDGGDATSATAVQAVIRQMVADEDRAKPLSDSQIMQRLADQGIVIARRTVAKYREALRIAPATLRKAHAAGR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=884334 ROL27_RS08235 WP_010929513.1 1763204..1764643(+) (rpoN/rpoN1) [Bordetella parapertussis strain 400431-b]
ATGCAAAATATTGTACCTATGACTCGTCCCGCGCTAGAACTTCGTCCTGGTCAACATTTGACGTTGACCCCTCAACTGCA
ACAGTCGATCCGGCTGTTGCAGCTGTCGTCGCTGGAGCTGGAGGCCGAACTGTCGCAGGCGCTGGCCGAGAATCCGTTGC
TGGAACGCGACGAAGATCCGGCGCGCGAGGAGCAGGCCGACCCCGAGCGCGAGGCCTCGGTCGAGGCCGACGAGGCGCCG
GATACCGAACTGCGCCTGGACGAGATGCCCGGTTCGGGCGGCGTTCTTCCCGACGACGACAGCGACCTGCCGCAGGCGGC
GCGCCCGGATACCCTGCGCGAACACCTGCTGGGGCAGCTGGCGCTGACGCGCGCCGCGCCGCGCGGTGCGGCGCTGTGCG
GTCTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGATTCGCCCCTGGAAGAGATCCTGACCTGGCTGCCGGCCGAG
GCGCAGGCCGACCTGGACGAGCTGCGCGCGGCGCTGGCATTGCTGCAGTCGTTCGATCCGGCAGGCATCGGCGCGCGCGA
CATGGCCGAATGCCTGCAACTGCAGTTGCGCCAGCCCGATCCGGCGCGCCTGCCCGAGGCGGCCGATGCCGAGGTGCTGG
CCTGTGCGCGGCGCATCTGCGCGCAGCATCTGCCCTTGCTGGCCACCGGCAACGTGGCCCGCGTGCGCGAGGCCGTGCAA
TGCGACGAGGCGGTCGTGCGCGCCGCGCATGCGCTGATCCTGCGCCTGGAACCGCGGCCGGGCCGCGCCTGGACGGTGCC
GGCGGCCGATTACGCCATTCCCGACGTGATCGTGCGCCGTACCCGCCAGGGCTGGCAGGCGGTGCTCAACAGCGCCGTCG
TGCCGCGGCTGCATGTCAATGCCGTCTATGCGCAAGTGCTGGGCGGCCAGCGCCAGGGCGGCAACGCCGGCCTGCAGGCG
CAGCTGCAACAGGCGCGCTGGATGATACGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGCGTGGCGCAGGCGATCGT
GGCGCATCAGCAGGCGTTCTTCACCCAGGGGCCGGCGGCCATGCGGCCGCTGATCCTCAAGGACATCGCCGGCGAACTGG
ATCTGCACGAATCGACCATTTCGCGCGCGACGACGCAGAAGTACATGCTCACACCGTTCGGTACGCTGGAGCTCAAGCGC
TTCTTTGGCGCGGGCGTGTCCACCGATGGCGGAGACGCCACCTCGGCCACCGCGGTGCAGGCGGTGATCCGCCAGATGGT
GGCCGACGAGGATCGCGCCAAGCCCTTGTCGGACAGCCAGATCATGCAGCGCCTGGCCGACCAGGGCATCGTCATTGCCC
GCCGCACGGTGGCCAAGTACCGCGAGGCGCTGCGGATTGCGCCAGCGACGCTGCGCAAGGCCCACGCCGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.221

100

0.509