Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   RNI18_RS20230 Genome accession   NZ_CP134775
Coordinates   4029637..4030245 (-) Length   202 a.a.
NCBI ID   WP_003153521.1    Uniprot ID   -
Organism   Bacillus velezensis strain KB21     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4024637..4035245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS20210 (RNI18_20210) dnaD 4024874..4025572 (+) 699 WP_003153516.1 DNA replication protein DnaD -
  RNI18_RS20215 (RNI18_20215) nth 4025591..4026250 (+) 660 WP_015388079.1 endonuclease III -
  RNI18_RS20220 (RNI18_20220) - 4026247..4026738 (+) 492 WP_046559838.1 YpoC family protein -
  RNI18_RS20225 (RNI18_20225) - 4026815..4029598 (-) 2784 WP_046559837.1 PBP1A family penicillin-binding protein -
  RNI18_RS20230 (RNI18_20230) recU 4029637..4030245 (-) 609 WP_003153521.1 Holliday junction resolvase RecU Machinery gene
  RNI18_RS20235 (RNI18_20235) - 4030287..4031249 (+) 963 WP_046559836.1 DUF2515 domain-containing protein -
  RNI18_RS20240 (RNI18_20240) sspM 4031293..4031397 (-) 105 WP_003153525.1 acid-soluble spore protein SspM -
  RNI18_RS20245 (RNI18_20245) - 4031603..4031848 (+) 246 WP_003153527.1 DUF5446 family protein -
  RNI18_RS20250 (RNI18_20250) - 4031894..4032262 (+) 369 WP_003153529.1 YppE family protein -
  RNI18_RS20255 (RNI18_20255) - 4032301..4032483 (-) 183 WP_003153532.1 YppF family protein -
  RNI18_RS20260 (RNI18_20260) - 4032669..4033103 (+) 435 WP_003153534.1 YppG family protein -
  RNI18_RS20265 (RNI18_20265) - 4033133..4033546 (-) 414 WP_003153535.1 hypothetical protein -
  RNI18_RS20270 (RNI18_20270) - 4033685..4034191 (+) 507 WP_003153536.1 PTS glucose transporter subunit IIA -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=883626 RNI18_RS20230 WP_003153521.1 4029637..4030245(-) (recU) [Bacillus velezensis strain KB21]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=883626 RNI18_RS20230 WP_003153521.1 4029637..4030245(-) (recU) [Bacillus velezensis strain KB21]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.955

98.515

0.896