Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   RNI18_RS19165 Genome accession   NZ_CP134775
Coordinates   3783666..3784466 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain KB21     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 3778666..3789466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS19145 (RNI18_19145) - 3778788..3779633 (+) 846 WP_015388017.1 TlyA family RNA methyltransferase -
  RNI18_RS19150 (RNI18_19150) ahrC 3779730..3780179 (+) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  RNI18_RS19155 (RNI18_19155) recN 3780211..3781941 (+) 1731 WP_207579556.1 DNA repair protein RecN Machinery gene
  RNI18_RS19160 (RNI18_19160) spoIVB 3782105..3783385 (+) 1281 WP_007408360.1 SpoIVB peptidase -
  RNI18_RS19165 (RNI18_19165) spo0A 3783666..3784466 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  RNI18_RS19170 (RNI18_19170) alr 3784612..3785793 (-) 1182 WP_015388018.1 alanine racemase -
  RNI18_RS19175 (RNI18_19175) - 3785969..3786181 (-) 213 WP_014305392.1 spore germination protein -
  RNI18_RS19180 (RNI18_19180) - 3786307..3786519 (-) 213 WP_003153183.1 spore germination protein -
  RNI18_RS19185 (RNI18_19185) - 3786645..3787763 (-) 1119 WP_058906708.1 NADH-dependent flavin oxidoreductase -
  RNI18_RS19190 (RNI18_19190) - 3787857..3787994 (-) 138 WP_224979422.1 hypothetical protein -
  RNI18_RS19195 (RNI18_19195) - 3788285..3788917 (-) 633 WP_014418348.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=883625 RNI18_RS19165 WP_003153177.1 3783666..3784466(+) (spo0A) [Bacillus velezensis strain KB21]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=883625 RNI18_RS19165 WP_003153177.1 3783666..3784466(+) (spo0A) [Bacillus velezensis strain KB21]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCCC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97