Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RNI18_RS18660 Genome accession   NZ_CP134775
Coordinates   3698431..3699198 (+) Length   255 a.a.
NCBI ID   WP_046559881.1    Uniprot ID   -
Organism   Bacillus velezensis strain KB21     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3693431..3704198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS18630 (RNI18_18630) - 3693714..3695846 (+) 2133 WP_046559882.1 HD family phosphohydrolase -
  RNI18_RS18635 (RNI18_18635) ybeY 3695847..3696320 (+) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  RNI18_RS18640 (RNI18_18640) - 3696301..3696675 (+) 375 WP_003152974.1 diacylglycerol kinase family protein -
  RNI18_RS18645 (RNI18_18645) - 3696799..3697209 (+) 411 WP_003152976.1 cytidine deaminase -
  RNI18_RS18650 (RNI18_18650) era 3697190..3698095 (+) 906 WP_003152978.1 GTPase Era -
  RNI18_RS18655 (RNI18_18655) - 3698251..3698394 (+) 144 WP_007408286.1 YqzL family protein -
  RNI18_RS18660 (RNI18_18660) recO 3698431..3699198 (+) 768 WP_046559881.1 DNA repair protein RecO Machinery gene
  RNI18_RS18665 (RNI18_18665) glyQ 3699500..3700387 (+) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  RNI18_RS18670 (RNI18_18670) glyS 3700380..3702419 (+) 2040 WP_014305438.1 glycine--tRNA ligase subunit beta -
  RNI18_RS18675 (RNI18_18675) ccpN 3702520..3703158 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  RNI18_RS18680 (RNI18_18680) - 3703184..3703996 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29263.92 Da        Isoelectric Point: 8.7950

>NTDB_id=883615 RNI18_RS18660 WP_046559881.1 3698431..3699198(+) (recO) [Bacillus velezensis strain KB21]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQKTKNEIKQVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=883615 RNI18_RS18660 WP_046559881.1 3698431..3699198(+) (recO) [Bacillus velezensis strain KB21]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACATTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGTAGCGTCTCGTTAAAACAGAAAA
CCAAAAACGAAATTAAACAGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89