Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   RNI18_RS12825 Genome accession   NZ_CP134775
Coordinates   2579230..2580375 (-) Length   381 a.a.
NCBI ID   WP_088037228.1    Uniprot ID   -
Organism   Bacillus velezensis strain KB21     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2574230..2585375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS12780 (RNI18_12780) speE 2574522..2575352 (+) 831 WP_003151052.1 spermidine synthase -
  RNI18_RS12785 (RNI18_12785) speB 2575412..2576284 (+) 873 WP_003151054.1 agmatinase -
  RNI18_RS12790 (RNI18_12790) - 2576454..2576672 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  RNI18_RS12795 (RNI18_12795) - 2577263..2577775 (-) 513 WP_032859656.1 stage II sporulation protein M -
  RNI18_RS12800 (RNI18_12800) - 2577819..2578100 (-) 282 WP_014306021.1 hypothetical protein -
  RNI18_RS12810 (RNI18_12810) - 2578508..2578711 (-) 204 WP_003151062.1 hypothetical protein -
  RNI18_RS12815 (RNI18_12815) - 2578725..2578928 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  RNI18_RS12820 (RNI18_12820) - 2579127..2579246 (-) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  RNI18_RS12825 (RNI18_12825) rapF 2579230..2580375 (-) 1146 WP_088037228.1 tetratricopeptide repeat protein Regulator
  RNI18_RS12830 (RNI18_12830) - 2580667..2581095 (+) 429 WP_207579577.1 DUF1934 domain-containing protein -
  RNI18_RS12835 (RNI18_12835) argS 2581092..2582762 (+) 1671 WP_207579576.1 arginine--tRNA ligase -
  RNI18_RS12840 (RNI18_12840) narK 2582897..2584087 (+) 1191 WP_044052814.1 nitrate transporter NarK -
  RNI18_RS12845 (RNI18_12845) - 2584185..2584901 (+) 717 WP_048367767.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45325.46 Da        Isoelectric Point: 4.9264

>NTDB_id=883590 RNI18_RS12825 WP_088037228.1 2579230..2580375(-) (rapF) [Bacillus velezensis strain KB21]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=883590 RNI18_RS12825 WP_088037228.1 2579230..2580375(-) (rapF) [Bacillus velezensis strain KB21]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTATCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.541

100

0.745
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53