Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RNI18_RS09370 Genome accession   NZ_CP134775
Coordinates   1920223..1920660 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain KB21     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1915223..1925660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNI18_RS09355 (RNI18_09355) - 1918081..1918443 (-) 363 WP_014304320.1 helix-turn-helix domain-containing protein -
  RNI18_RS09360 (RNI18_09360) hxlA 1918675..1919310 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  RNI18_RS09365 (RNI18_09365) hxlB 1919307..1919864 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  RNI18_RS09370 (RNI18_09370) nucA/comI 1920223..1920660 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RNI18_RS09375 (RNI18_09375) nin/comJ 1920682..1921080 (+) 399 WP_043866822.1 competence protein ComJ Regulator
  RNI18_RS09380 (RNI18_09380) - 1921121..1921339 (-) 219 Protein_1838 family 1 glycosylhydrolase -
  RNI18_RS20825 - 1921371..1921616 (-) 246 WP_109567708.1 family 1 glycosylhydrolase -
  RNI18_RS09385 (RNI18_09385) - 1921558..1921833 (-) 276 WP_369719109.1 YckD family protein -
  RNI18_RS09390 (RNI18_09390) - 1921950..1922384 (-) 435 WP_021494203.1 RDD family protein -
  RNI18_RS09395 (RNI18_09395) - 1922399..1923535 (-) 1137 WP_048368274.1 zinc-dependent alcohol dehydrogenase -
  RNI18_RS09400 (RNI18_09400) - 1923600..1924802 (-) 1203 WP_025853783.1 GTP-binding protein -
  RNI18_RS09405 (RNI18_09405) - 1924955..1925080 (+) 126 Protein_1844 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=883579 RNI18_RS09370 WP_003156588.1 1920223..1920660(+) (nucA/comI) [Bacillus velezensis strain KB21]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=883579 RNI18_RS09370 WP_003156588.1 1920223..1920660(+) (nucA/comI) [Bacillus velezensis strain KB21]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCAATAAAAGAAGGGCATTCCAGCATTTGCACCATTGATAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641