Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RM152_RS16465 Genome accession   NZ_CP134753
Coordinates   3540484..3541866 (-) Length   460 a.a.
NCBI ID   WP_031593287.1    Uniprot ID   -
Organism   Pantoea agglomerans strain AR5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3535484..3546866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RM152_RS16440 (RM152_16440) - 3536259..3536423 (+) 165 WP_109652447.1 YhfG family protein -
  RM152_RS16445 (RM152_16445) - 3536436..3537038 (+) 603 WP_069025796.1 putative adenosine monophosphate-protein transferase Fic -
  RM152_RS16450 (RM152_16450) - 3537019..3537915 (-) 897 WP_010253616.1 LysR family transcriptional regulator -
  RM152_RS16455 (RM152_16455) - 3538120..3539121 (+) 1002 WP_045140797.1 zinc-binding alcohol dehydrogenase family protein -
  RM152_RS16460 (RM152_16460) nadR 3539167..3540408 (-) 1242 WP_010253612.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  RM152_RS16465 (RM152_16465) radA 3540484..3541866 (-) 1383 WP_031593287.1 DNA repair protein RadA Machinery gene
  RM152_RS16470 (RM152_16470) serB 3541880..3542857 (-) 978 WP_115765288.1 phosphoserine phosphatase -
  RM152_RS16475 (RM152_16475) - 3542965..3543615 (+) 651 WP_010253606.1 YtjB family periplasmic protein -
  RM152_RS16480 (RM152_16480) yjjG 3544282..3544962 (-) 681 WP_010671712.1 pyrimidine 5'-nucleotidase -
  RM152_RS16485 (RM152_16485) rimI 3544981..3545421 (-) 441 WP_115765513.1 ribosomal protein S18-alanine N-acetyltransferase -
  RM152_RS16490 (RM152_16490) - 3545393..3545803 (-) 411 WP_115765514.1 DNA polymerase III subunit psi -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49307.93 Da        Isoelectric Point: 7.4220

>NTDB_id=883343 RM152_RS16465 WP_031593287.1 3540484..3541866(-) (radA) [Pantoea agglomerans strain AR5]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGSPGAGKSTLLLQVMCRLSEGMKTLYVTGEESLQQVAMRAHRLGLPTENVNMLSETSIE
QICLIAEQEQPQLMVIDSIQVMHMAEIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEVTSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERIAEAAKHGFKRAIVPAGNAPKKPIAGMQVFSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=883343 RM152_RS16465 WP_031593287.1 3540484..3541866(-) (radA) [Pantoea agglomerans strain AR5]
TTGGCCAAAGCGGCAAAACGCGCATTTGTCTGTAACGAATGTGGCGCAGATTACCCACGCTGGCAGGGGCAGTGCAGCGC
CTGTCACGCCTGGAACACCATCACTGAGGTGCGCATTGCCGCCTCGCCCGCGGCTGCGCGTAACGAGCGCCTGAGTGGCT
ATGCCGGCAGCGCCGGAACCAGCCGGGTGCAGAAGCTCTCTGAAATCAGTTTAGAGGCGTTACCGCGCTTCTCTACCAGC
TTCAAAGAGTTTGACCGGGTGCTGGGCGGCGGCGTGGTGCCGGGCAGCGCCATTCTGATTGGCGGCAGCCCCGGCGCAGG
CAAATCCACGCTGCTGCTGCAGGTGATGTGCCGGTTGTCAGAGGGGATGAAAACCCTGTATGTCACCGGTGAAGAGTCAC
TACAACAGGTGGCGATGCGCGCCCATCGTCTGGGATTACCTACCGAAAATGTGAATATGCTGTCGGAAACCAGCATTGAG
CAGATCTGCCTGATCGCCGAACAGGAGCAGCCGCAACTGATGGTGATCGACTCGATCCAGGTGATGCACATGGCGGAGAT
CCAGTCATCGCCGGGCAGCGTCGCCCAGGTGCGTGAAACCGCCGCTTACCTGACGCGCTTCGCCAAGACGCGCGGCGTGG
CGATCATCATGGTCGGTCACGTCACCAAAGATGGCTCCCTGGCCGGACCGAAAGTACTGGAGCACTGTATCGACTGTTCG
GTACTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGTCATAAAAACCGCTTCGGTGCGGTTAATGAACT
GGGTGTGTTTGCCATGACCGAGCAGGGCATGCGTGAAGTCAGCAACCCGTCAGCTATTTTCCTGTCGCGCGGCGAAGAGG
TCACCTCGGGCAGTTCGGTGATGGTGCTGTGGGAAGGAACGCGACCGCTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGGCAATCCACGCCGCGTGGCAGTGGGTCTGGAGCAGAACCGTCTGGCGATTCTGCTGGCGGTGCTGCACCG
GCACGGCGGATTGCAGATGGCGGATCAGGATGTGTTCGTTAACGTGGTCGGCGGCGTCAAAGTCACTGAAACCAGTGCTG
ACCTGGCGCTGATGCTGTCGATGGTGTCGAGCCTGCGCGATCGTCCGTTGCCGCAGGATCTGGTGATCTTCGGCGAAGTG
GGACTGGCGGGCGAAATCCGTCCGGTGCCCAGCGGTCAGGAGCGCATCGCTGAAGCGGCGAAGCATGGCTTCAAACGCGC
TATTGTACCGGCAGGGAATGCACCGAAAAAACCGATTGCCGGGATGCAGGTTTTCAGCGCGAAGAAGCTGGCCGATGCGC
TGGCGATTCTGGACGATCTGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)

AAA domain (AAA_25)

(87-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.373

100

0.485
  radA Streptococcus mitis NCTC 12261

44.82

96.522

0.433
  radA Streptococcus pneumoniae Rx1

45.244

93.696

0.424
  radA Streptococcus pneumoniae D39

45.244

93.696

0.424
  radA Streptococcus pneumoniae R6

45.244

93.696

0.424
  radA Streptococcus pneumoniae TIGR4

45.244

93.696

0.424
  radA Streptococcus mitis SK321

45.244

93.696

0.424