Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RJY17_RS12955 Genome accession   NZ_CP134692
Coordinates   2492016..2492453 (-) Length   145 a.a.
NCBI ID   WP_032867344.1    Uniprot ID   -
Organism   Bacillus velezensis strain B5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2487016..2497453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJY17_RS12930 (RJY17_12930) - 2488036..2489172 (+) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -
  RJY17_RS12935 (RJY17_12935) - 2489187..2489621 (+) 435 WP_007409358.1 RDD family protein -
  RJY17_RS12940 (RJY17_12940) - 2489738..2490016 (+) 279 WP_263612330.1 YckD family protein -
  RJY17_RS12945 (RJY17_12945) - 2490120..2491556 (+) 1437 WP_007409356.1 glycoside hydrolase family 1 protein -
  RJY17_RS12950 (RJY17_12950) nin/comJ 2491597..2491995 (-) 399 WP_015239128.1 competence protein ComJ Regulator
  RJY17_RS12955 (RJY17_12955) nucA/comI 2492016..2492453 (-) 438 WP_032867344.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RJY17_RS12960 (RJY17_12960) hxlB 2492811..2493368 (-) 558 WP_015239129.1 6-phospho-3-hexuloisomerase -
  RJY17_RS12965 (RJY17_12965) hxlA 2493365..2494000 (-) 636 WP_094032058.1 3-hexulose-6-phosphate synthase -
  RJY17_RS12970 (RJY17_12970) - 2494232..2494594 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16094.41 Da        Isoelectric Point: 9.0073

>NTDB_id=882897 RJY17_RS12955 WP_032867344.1 2492016..2492453(-) (nucA/comI) [Bacillus velezensis strain B5]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSHYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=882897 RJY17_RS12955 WP_032867344.1 2492016..2492453(-) (nucA/comI) [Bacillus velezensis strain B5]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTCATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641