Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RIK65_RS01760 Genome accession   NZ_CP134642
Coordinates   361081..362514 (-) Length   477 a.a.
NCBI ID   WP_063143582.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 30A:C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356081..367514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIK65_RS01730 (RIK65_01730) elbB 357615..358268 (+) 654 WP_213328942.1 isoprenoid biosynthesis glyoxalase ElbB -
  RIK65_RS01735 (RIK65_01735) mtgA 358265..358990 (+) 726 WP_311391678.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RIK65_RS01740 (RIK65_01740) npr 359028..359300 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  RIK65_RS01745 (RIK65_01745) rapZ 359297..360151 (-) 855 WP_014885397.1 RNase adapter RapZ -
  RIK65_RS01750 (RIK65_01750) ptsN 360197..360688 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RIK65_RS01755 (RIK65_01755) hpf 360771..361058 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  RIK65_RS01760 (RIK65_01760) rpoN/rpoN1 361081..362514 (-) 1434 WP_063143582.1 RNA polymerase factor sigma-54 Machinery gene
  RIK65_RS01765 (RIK65_01765) lptB 362562..363287 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  RIK65_RS01770 (RIK65_01770) lptA 363294..363848 (-) 555 WP_023333608.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RIK65_RS01775 (RIK65_01775) lptC 363817..364392 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  RIK65_RS01780 (RIK65_01780) kdsC 364389..364955 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RIK65_RS01785 (RIK65_01785) kdsD 364970..365956 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  RIK65_RS01790 (RIK65_01790) - 365970..366947 (-) 978 WP_039260412.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=882602 RIK65_RS01760 WP_063143582.1 361081..362514(-) (rpoN/rpoN1) [Enterobacter asburiae strain 30A:C]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=882602 RIK65_RS01760 WP_063143582.1 361081..362514(-) (rpoN/rpoN1) [Enterobacter asburiae strain 30A:C]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCTCAGGATACAGAAGCGCTCGACACCGCGGATGCTCTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTATCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCACGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTGATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCTAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482