Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   RL451_RS09585 Genome accession   NZ_CP134617
Coordinates   1938544..1939905 (+) Length   453 a.a.
NCBI ID   WP_278198198.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IPLA16     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 1939902..1960557 1938544..1939905 flank -3


Gene organization within MGE regions


Location: 1938544..1960557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RL451_RS09585 (RL451_09585) ccrA 1938544..1939905 (+) 1362 WP_278198198.1 cassette chromosome recombinase CcrA Machinery gene
  RL451_RS09590 (RL451_09590) ccrB 1939902..1941527 (+) 1626 WP_278198199.1 cassette chromosome recombinase CcrB Machinery gene
  RL451_RS09595 (RL451_09595) - 1942047..1942397 (+) 351 WP_278198200.1 SAUGI family uracil-DNA glycosylase inhibitor -
  RL451_RS09600 (RL451_09600) - 1942484..1942795 (+) 312 WP_278198201.1 DUF960 family protein -
  RL451_RS09605 (RL451_09605) - 1942810..1943313 (+) 504 WP_278198202.1 DUF1643 domain-containing protein -
  RL451_RS09610 (RL451_09610) - 1943327..1943548 (+) 222 WP_001836575.1 hypothetical protein -
  RL451_RS09615 (RL451_09615) - 1943711..1944121 (+) 411 WP_134293269.1 DUF2871 domain-containing protein -
  RL451_RS09620 (RL451_09620) - 1944793..1945290 (+) 498 WP_017175155.1 GNAT family N-acetyltransferase -
  RL451_RS09625 (RL451_09625) - 1945348..1945571 (+) 224 Protein_1858 IS5/IS1182 family transposase -
  RL451_RS09630 (RL451_09630) - 1945600..1946802 (-) 1203 WP_149809826.1 restriction endonuclease subunit S -
  RL451_RS09635 (RL451_09635) - 1947122..1947607 (-) 486 WP_278198203.1 GNAT family N-acetyltransferase -
  RL451_RS09640 (RL451_09640) - 1947871..1948545 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  RL451_RS09645 (RL451_09645) - 1948611..1948892 (-) 282 WP_001802813.1 hypothetical protein -
  RL451_RS09650 (RL451_09650) - 1948917..1949174 (-) 258 WP_001802814.1 hypothetical protein -
  RL451_RS09655 (RL451_09655) - 1949362..1949877 (+) 516 WP_000377077.1 hypothetical protein -
  RL451_RS09660 (RL451_09660) merB 1950072..1950722 (-) 651 WP_000790334.1 organomercurial lyase MerB -
  RL451_RS09665 (RL451_09665) merA 1950804..1952447 (-) 1644 WP_000193219.1 mercury(II) reductase -
  RL451_RS09670 (RL451_09670) - 1952505..1952843 (-) 339 WP_000968575.1 hypothetical protein -
  RL451_RS09675 (RL451_09675) - 1952978..1953658 (-) 681 WP_000010351.1 cytochrome c biogenesis CcdA family protein -
  RL451_RS09680 (RL451_09680) - 1953655..1954008 (-) 354 WP_000746201.1 TlpA family protein disulfide reductase -
  RL451_RS09685 (RL451_09685) merR 1954025..1954432 (-) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  RL451_RS09690 (RL451_09690) - 1954732..1956078 (-) 1347 WP_000163428.1 dihydrolipoyl dehydrogenase family protein -
  RL451_RS09695 (RL451_09695) - 1956361..1957035 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  RL451_RS09700 (RL451_09700) - 1957077..1957487 (-) 411 WP_331385785.1 JAB domain-containing protein -
  RL451_RS09705 (RL451_09705) - 1957508..1958014 (-) 507 WP_000849085.1 DUF1643 domain-containing protein -
  RL451_RS09710 (RL451_09710) - 1958030..1958341 (-) 312 WP_001083108.1 DUF960 family protein -
  RL451_RS09715 (RL451_09715) - 1958437..1958775 (-) 339 WP_000171557.1 SAUGI family uracil-DNA glycosylase inhibitor -
  RL451_RS09720 (RL451_09720) ccrC 1958881..1960557 (-) 1677 WP_002504707.1 cassette chromosome recombinase CcrC -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 53178.07 Da        Isoelectric Point: 9.8894

>NTDB_id=882368 RL451_RS09585 WP_278198198.1 1938544..1939905(+) (ccrA) [Staphylococcus aureus strain IPLA16]
MEKAIAYVRQSTLKQQSLATQKSLIIDTAKQHGWSNVTFYDDKKTGRHTKRSGYQKMVEVITSGECKVLCCYRLNRLHRN
FKNAIQFFEICKKHHVTIISVNDGYFDLSKEFDCFRLNILMSLAEMESNNISEQTRNGIREKAKQGKLITTHAPFGYHYS
KGHFIVNAEEAPTIKAVYRWYLQGLGYKKISQKLDKDMNFISRKPYQVRNILLNPNYCGRVINKYGTFNDIVPPIINVDT
FEEAQERRIHKQHNRSNSRNKLKRRIKCPYCQSTLTNLTIKKEHRSLRYYVCPKNMNEAYHTCPFKGINAQMIETEVLNT
CKAYCNNQSFHDKLNETILKVLKYQQMKHRQSHLTQEQLIEKLAQNQIDIETFKHLSAGSESEERYSNYSHKQIAEAIRH
VIKDKLTLENIAPLIDDIKITQTKQLKEIYFKNSRLNIVEQSHLITPERNEVI

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=882368 RL451_RS09585 WP_278198198.1 1938544..1939905(+) (ccrA) [Staphylococcus aureus strain IPLA16]
ATGGAAAAAGCCATTGCTTATGTACGTCAAAGTACACTAAAACAACAAAGCTTAGCGACTCAGAAATCCCTCATTATAGA
CACTGCTAAGCAACATGGATGGTCCAATGTAACATTTTATGATGATAAAAAAACAGGTCGACATACAAAGCGTTCAGGCT
ATCAAAAAATGGTAGAAGTGATTACATCTGGAGAGTGTAAAGTATTATGTTGCTATCGGCTAAATCGGCTCCATCGTAAT
TTCAAAAATGCTATACAATTCTTTGAAATTTGTAAAAAGCATCACGTCACAATCATCAGTGTTAACGATGGATATTTTGA
TTTGTCTAAAGAGTTTGATTGCTTTCGCTTAAACATACTCATGAGTCTAGCAGAAATGGAATCGAACAACATCAGCGAAC
AAACACGAAATGGGATAAGAGAAAAAGCCAAACAAGGTAAGTTGATTACAACACACGCCCCTTTTGGTTATCACTACAGC
AAAGGTCACTTTATTGTAAATGCAGAAGAAGCACCGACAATCAAAGCCGTTTATCGTTGGTATTTACAAGGACTTGGTTA
CAAGAAAATTTCTCAAAAGCTGGATAAAGATATGAATTTTATTTCTCGTAAGCCCTATCAAGTTAGAAATATTCTCTTGA
ACCCTAACTATTGTGGTCGTGTCATTAATAAATATGGAACGTTTAATGATATCGTTCCACCTATTATTAATGTTGATACG
TTTGAGGAAGCACAAGAGCGTCGTATTCATAAACAACACAATCGATCCAATTCTAGAAATAAGTTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAACATCGTTCTCTACGTTACTATGTCTGTCCTA
AAAATATGAATGAAGCTTATCATACGTGTCCATTTAAAGGTATCAATGCGCAAATGATTGAAACAGAGGTCCTCAACACT
TGTAAAGCATATTGTAACAATCAGTCATTTCATGACAAATTAAATGAGACAATACTAAAAGTTCTAAAATACCAACAAAT
GAAACACAGACAATCACATCTAACACAAGAACAACTCATCGAAAAGCTAGCACAAAATCAAATTGATATCGAGACGTTTA
AACATTTATCTGCTGGCTCTGAAAGTGAAGAACGTTACTCTAATTATTCACATAAACAAATTGCCGAAGCAATCCGTCAT
GTAATCAAAGATAAATTGACATTAGAAAACATAGCACCTTTGATTGATGACATTAAGATAACTCAAACTAAACAATTAAA
AGAGATCTACTTTAAAAACAGTCGACTTAATATCGTTGAACAATCACATTTAATAACTCCTGAAAGGAATGAAGTCATAT
GA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

55.53

97.792

0.543
  ccrA Staphylococcus aureus N315

51.919

97.792

0.508