Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WMZ02_RS14205 Genome accession   NZ_CP150864
Coordinates   3013223..3014689 (+) Length   488 a.a.
NCBI ID   WP_005484866.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain NJIFDCVp7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3008223..3019689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMZ02_RS14180 (WMZ02_14180) - 3009343..3010308 (+) 966 WP_005455122.1 calcium/sodium antiporter -
  WMZ02_RS14185 (WMZ02_14185) kdsD 3010345..3011316 (+) 972 WP_020841311.1 arabinose-5-phosphate isomerase KdsD -
  WMZ02_RS14190 (WMZ02_14190) lptC 3011407..3011970 (+) 564 WP_346338061.1 LPS export ABC transporter periplasmic protein LptC -
  WMZ02_RS14195 (WMZ02_14195) lptA 3011951..3012445 (+) 495 WP_005467803.1 lipopolysaccharide transport periplasmic protein LptA -
  WMZ02_RS14200 (WMZ02_14200) lptB 3012447..3013172 (+) 726 WP_005467801.1 LPS export ABC transporter ATP-binding protein -
  WMZ02_RS14205 (WMZ02_14205) rpoN/rpoN1 3013223..3014689 (+) 1467 WP_005484866.1 RNA polymerase factor sigma-54 Machinery gene
  WMZ02_RS14210 (WMZ02_14210) hpf 3014714..3015001 (+) 288 WP_005467451.1 ribosome hibernation promoting factor -
  WMZ02_RS14215 (WMZ02_14215) ptsN 3015004..3015450 (+) 447 WP_005467453.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WMZ02_RS14220 (WMZ02_14220) rapZ 3015465..3016328 (+) 864 WP_005468663.1 RNase adapter RapZ -
  WMZ02_RS14225 (WMZ02_14225) - 3016331..3016606 (+) 276 WP_005468661.1 HPr family phosphocarrier protein -
  WMZ02_RS14230 (WMZ02_14230) mgtE 3016825..3018180 (+) 1356 WP_005468659.1 magnesium transporter -
  WMZ02_RS14235 (WMZ02_14235) pmbA 3018257..3019600 (-) 1344 WP_005459755.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54838.66 Da        Isoelectric Point: 4.3441

>NTDB_id=880799 WMZ02_RS14205 WP_005484866.1 3013223..3014689(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain NJIFDCVp7]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLEVEEGHEEPQANGEDKSALETADNSANEPTEIE
VPDSSDVIEKSEISSELEIDTTWDDVYSANTGSTGLALDDDMPVYQGETTESLHDYLMWQLDLTPFSETDRTIAIAIIDA
IDDYGYLTLSPEEIHESFDNEDIELDEVEAVRKRIQQFDPLGVASRNLQECLLLQLATFPEDTPWLAEAKMILADHIDHL
GNRDYKLVIKETKLKEADLREVLKLIQQLDPRPGSRITPDDTEYVIPDVSVFKDHGKWTVSINPDSIPKLKVNQQYAQLG
KGNSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDVALAVDMHESTISRVTTQKF
MHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAP
SSQRKRLL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=880799 WMZ02_RS14205 WP_005484866.1 3013223..3014689(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain NJIFDCVp7]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCACAGTTGCAACAAGCGATTCGCTTGCTGCA
GTTGTCGACTCTCGATCTTCAACAAGAAATCCAAGAAGCGTTGGACTCAAACCCACTATTGGAAGTTGAAGAAGGCCACG
AAGAACCTCAAGCAAATGGTGAAGATAAATCAGCACTCGAAACTGCTGACAACAGTGCCAACGAGCCAACAGAAATCGAA
GTTCCTGATAGTTCTGACGTCATCGAAAAGTCCGAAATCAGCTCAGAGCTTGAAATCGACACAACTTGGGATGACGTCTA
CAGCGCAAATACGGGCAGTACCGGTTTAGCGTTAGACGATGATATGCCGGTCTATCAAGGCGAAACCACAGAATCGCTAC
ACGATTACCTGATGTGGCAACTCGACTTAACGCCTTTCAGTGAAACCGACCGTACGATTGCCATTGCCATCATTGATGCG
ATTGACGATTACGGCTATCTGACCCTATCTCCTGAAGAGATTCACGAAAGCTTCGACAACGAAGATATTGAGTTGGATGA
AGTCGAAGCCGTGCGCAAACGCATCCAACAGTTTGATCCGCTTGGTGTGGCATCGCGGAACCTGCAAGAATGCTTACTCC
TGCAACTTGCAACCTTCCCAGAAGACACGCCTTGGCTTGCGGAAGCCAAAATGATTCTTGCCGATCACATCGATCACCTT
GGTAATCGCGATTACAAACTGGTCATCAAAGAAACTAAGCTAAAAGAAGCGGATCTACGCGAAGTATTAAAACTGATCCA
ACAGTTAGATCCTCGTCCGGGCAGCCGCATTACCCCAGACGACACAGAATACGTCATTCCGGACGTATCGGTATTTAAAG
ATCATGGGAAATGGACCGTTTCCATCAACCCTGACAGCATTCCAAAGTTAAAAGTTAACCAACAATACGCTCAATTAGGC
AAAGGCAACAGTGCGGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCAAAATGGTTAATTAAGAGCTTAGAGAGCAG
AAACGAGACGCTACTCAAAGTTGCAAGATGTATTGTTGAACATCAACAAGATTTCTTCGAGTATGGTGAAGAAGCCATGA
AGCCAATGGTACTCAATGATGTAGCCTTGGCGGTGGACATGCATGAATCCACAATATCTCGTGTTACGACACAAAAGTTC
ATGCATACGCCACGTGGCATTTTTGAATTGAAGTACTTCTTCTCCAGCCACGTCAGTACAGACAATGGCGGAGAATGTTC
GTCAACAGCAATCCGCGCACTCATTAAGAAACTGGTCGCGGCAGAGAATACTGCTAAACCACTTAGTGACAGTAAGATTG
CTGCTCTTCTGGCTGACCAAGGGATTCAGGTCGCAAGACGTACGATAGCTAAATATCGAGAATCCTTGGGTATTGCCCCT
TCAAGTCAGCGTAAACGCCTACTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.444

100

0.459