Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   RKV26_RS00485 Genome accession   NZ_CP134532
Coordinates   63534..65162 (-) Length   542 a.a.
NCBI ID   WP_001186600.1    Uniprot ID   Q4JFB1
Organism   Staphylococcus aureus strain KNIH_6268     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 63465..92317 63534..65162 within 0
SCCmec 45168..70594 63534..65162 within 0


Gene organization within MGE regions


Location: 45168..92317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKV26_RS00385 mecA 45168..47177 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  RKV26_RS00390 mecR1 47274..49031 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  RKV26_RS00395 mecI 49031..49402 (+) 372 WP_267814210.1 mecA-type methicillin resistance repressor MecI -
  RKV26_RS00400 psm-mec 49487..49555 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  RKV26_RS00405 - 49910..51018 (+) 1109 Protein_39 ROK family protein -
  RKV26_RS00410 cstB 51132..52466 (-) 1335 Protein_40 persulfide dioxygenase-sulfurtransferase CstB -
  RKV26_RS00415 - 52499..53563 (-) 1065 WP_267814212.1 DsrE/DsrF/DrsH-like family protein -
  RKV26_RS00420 cstR 53699..53959 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  RKV26_RS00425 - 53959..54597 (+) 639 Protein_43 sulfite exporter TauE/SafE family protein -
  RKV26_RS00430 - 54651..54710 (-) 60 Protein_44 DNA repair protein RadC -
  RKV26_RS00435 - 54942..55604 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  RKV26_RS14370 - 55996..56055 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  RKV26_RS00440 erm(A) 56138..56869 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  RKV26_RS00445 - 56995..57777 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  RKV26_RS00450 - 57928..58305 (-) 378 WP_000361059.1 DUF6262 family protein -
  RKV26_RS00455 - 58312..60204 (-) 1893 WP_001557544.1 site-specific integrase -
  RKV26_RS00460 - 60201..61286 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  RKV26_RS00465 - 61405..61722 (-) 318 WP_000659050.1 JAB domain-containing protein -
  RKV26_RS00470 - 61743..62249 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  RKV26_RS00475 - 62267..62578 (-) 312 WP_000700853.1 DUF960 family protein -
  RKV26_RS00480 - 62665..63015 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  RKV26_RS00485 ccrB 63534..65162 (-) 1629 WP_001186600.1 cassette chromosome recombinase CcrB Machinery gene
  RKV26_RS00490 ccrA 65184..66533 (-) 1350 WP_000815654.1 cassette chromosome recombinase CcrA Machinery gene
  RKV26_RS00495 cch1 66767..68554 (-) 1788 WP_001573627.1 cassette chromosome replicative helicase -
  RKV26_RS00500 ssb 68554..68868 (-) 315 WP_001573626.1 cassette chromosome ssDNA-binding protein -
  RKV26_RS00505 - 69149..70711 (-) 1563 WP_311163513.1 hypothetical protein -
  RKV26_RS00510 - 70774..71946 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  RKV26_RS00515 aph(2'')-Ia 72076..73515 (-) 1440 WP_001028144.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
  RKV26_RS00520 - 73516..73908 (-) 393 WP_000393259.1 GNAT family N-acetyltransferase -
  RKV26_RS00525 - 73965..75136 (-) 1172 Protein_64 IS256 family transposase -
  RKV26_RS00530 - 75667..76224 (-) 558 WP_311163762.1 ATP-binding protein -
  RKV26_RS00535 - 76131..76607 (-) 477 WP_311163514.1 hypothetical protein -
  RKV26_RS00540 - 76890..77069 (+) 180 WP_154284439.1 hypothetical protein -
  RKV26_RS00545 - 77323..78246 (+) 924 WP_267814216.1 hypothetical protein -
  RKV26_RS00550 istA 78351..79586 (+) 1236 WP_001215400.1 IS21 family transposase -
  RKV26_RS00555 istB 79598..80365 (+) 768 WP_086094926.1 IS21-like element helper ATPase IstB -
  RKV26_RS00560 - 80471..81316 (+) 846 WP_281961901.1 DUF4209 domain-containing protein -
  RKV26_RS00565 - 81354..82517 (+) 1164 WP_311163515.1 hypothetical protein -
  RKV26_RS00570 - 82955..83113 (+) 159 WP_195758897.1 hypothetical protein -
  RKV26_RS00575 selZ 83492..84268 (+) 777 WP_154284306.1 staphylococcal enterotoxin type Z -
  RKV26_RS00580 - 84453..85439 (-) 987 WP_000662036.1 tRNA-dihydrouridine synthase -
  RKV26_RS00585 - 85748..85951 (+) 204 WP_154284307.1 DUF6007 family protein -
  RKV26_RS00590 - 86000..86296 (+) 297 WP_154284308.1 TfoX/Sxy family protein -
  RKV26_RS00595 - 86505..87107 (+) 603 WP_154284322.1 hypothetical protein -
  RKV26_RS00600 - 87374..90526 (+) 3153 WP_209205686.1 ATP-binding protein -
  RKV26_RS00605 - 90601..91083 (+) 483 WP_154284310.1 hypothetical protein -
  RKV26_RS00610 - 91288..92274 (+) 987 WP_154284311.1 phosphatidylinositol-specific phospholipase C -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62769.00 Da        Isoelectric Point: 9.8480

>NTDB_id=880682 RKV26_RS00485 WP_001186600.1 63534..65162(-) (ccrB) [Staphylococcus aureus strain KNIH_6268]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKDGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRANVIEDYVMKQILEIVKSDKIIERVVARVNQENQVDCASLHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITYQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=880682 RKV26_RS00485 WP_001186600.1 63534..65162(-) (ccrB) [Staphylococcus aureus strain KNIH_6268]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTTTCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGATGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGTAAC
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACATGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCTCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACCAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTAATGTGATTGAAGATTATGTGATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAAATCATTGAACGTGTTG
TAGCACGTGTTAACCAAGAAAATCAAGTTGATTGTGCGTCACTTCATCACGATATCGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGTATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4JFB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.155

100

0.982