Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RKE53_RS21990 Genome accession   NZ_CP134413
Coordinates   4710984..4712105 (+) Length   373 a.a.
NCBI ID   WP_104395729.1    Uniprot ID   -
Organism   Microcystis aeruginosa NRERC-214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4705984..4717105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE53_RS21960 (RKE53_21960) - 4706282..4706896 (-) 615 Protein_4354 RNA polymerase subunit sigma-70 -
  RKE53_RS21965 (RKE53_21965) - 4707110..4707886 (-) 777 WP_104395733.1 M48 family metallopeptidase -
  RKE53_RS21970 (RKE53_21970) - 4707950..4708234 (-) 285 WP_104395732.1 hypothetical protein -
  RKE53_RS21975 (RKE53_21975) - 4708233..4708478 (+) 246 WP_104395731.1 hypothetical protein -
  RKE53_RS21980 (RKE53_21980) - 4708537..4709325 (+) 789 WP_002766121.1 ECF transporter S component -
  RKE53_RS21985 (RKE53_21985) ilvC 4709476..4710468 (-) 993 WP_104395730.1 ketol-acid reductoisomerase -
  RKE53_RS21990 (RKE53_21990) pilM 4710984..4712105 (+) 1122 WP_104395729.1 type IV pilus assembly protein PilM Machinery gene
  RKE53_RS21995 (RKE53_21995) - 4712115..4712885 (+) 771 WP_104395728.1 PilN domain-containing protein -
  RKE53_RS22000 (RKE53_22000) - 4712882..4713655 (+) 774 WP_104395727.1 pilus assembly protein -
  RKE53_RS22005 (RKE53_22005) - 4713729..4715825 (+) 2097 WP_104395726.1 secretin N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41672.87 Da        Isoelectric Point: 4.4414

>NTDB_id=880000 RKE53_RS21990 WP_104395729.1 4710984..4712105(+) (pilM) [Microcystis aeruginosa NRERC-214]
MVSFFKKLLTKKTQGIGLEITPERLNVAQIAKQKQNYKLVKYCSSDIPEGIFEEGKIVDPVALAELIQEVLKENKITRKR
VATAVPMRESIIRIIPIPSELDEEELRDLVLNHEANLYLPYPREEVDIDYQKLGYFQDEDGIEKVQVLLVATRREITDAY
METLQRAGLQVDVLEINSFALIRTIRDQLRQFSSNEATVIVDIEFDNTEIAIVVEGVPQFSRTVPIGTSQLQNAISRAMN
LPSSRSPEILLGMTIPTTAFDSLGTNTSASRTFTTAGIAALMRVLGELTDELRRSINFYLNQSDELEMEIVQLLLAGPGG
GLAQLDEYFTQRLSIPTMPIDPIAALNLDINQEIPNTQRPGLGTVLGLGLREV

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=880000 RKE53_RS21990 WP_104395729.1 4710984..4712105(+) (pilM) [Microcystis aeruginosa NRERC-214]
ATGGTAAGTTTTTTTAAGAAGCTATTGACAAAAAAAACTCAAGGTATTGGGTTAGAGATTACCCCAGAGCGCCTTAACGT
GGCTCAGATCGCCAAACAGAAACAAAATTATAAATTAGTTAAATATTGTTCGTCGGATATCCCAGAAGGCATCTTTGAGG
AGGGAAAAATCGTCGATCCCGTCGCTTTAGCGGAATTAATCCAAGAAGTCCTCAAGGAAAATAAAATTACTAGAAAACGA
GTCGCTACTGCTGTCCCCATGCGCGAGTCAATTATCCGGATTATCCCTATTCCCTCGGAATTGGACGAGGAGGAATTGCG
CGATCTGGTCTTAAATCATGAAGCTAATTTATACCTTCCCTATCCGCGGGAGGAAGTGGATATCGACTACCAAAAACTGG
GCTACTTTCAGGATGAAGACGGCATCGAAAAGGTGCAGGTTTTGCTGGTAGCTACTCGTCGGGAGATCACCGATGCCTAT
ATGGAAACCCTGCAACGGGCAGGATTACAGGTAGATGTGCTAGAAATTAATAGTTTTGCCCTAATTCGGACGATTCGCGA
TCAGTTGCGACAGTTTAGTTCCAATGAAGCTACCGTTATTGTCGATATAGAATTTGATAACACGGAAATTGCCATCGTGG
TGGAAGGCGTTCCCCAATTTTCCCGCACTGTCCCCATCGGCACTTCCCAGCTACAAAATGCCATTTCCAGGGCGATGAAT
TTGCCCAGTTCCCGCAGTCCTGAGATTCTTTTGGGGATGACAATCCCGACCACTGCCTTTGATAGTCTTGGCACCAATAC
CAGTGCTTCCAGAACCTTTACCACCGCCGGGATTGCCGCACTGATGCGGGTTTTAGGGGAATTAACCGATGAATTGCGGC
GATCAATTAATTTCTATCTTAACCAAAGTGATGAACTGGAAATGGAAATTGTCCAGTTACTTTTAGCCGGTCCCGGGGGC
GGTTTAGCACAACTAGATGAATATTTTACTCAACGTCTTAGCATCCCTACCATGCCCATCGATCCGATCGCAGCCCTGAA
CTTAGATATCAATCAAGAGATTCCTAATACCCAAAGACCGGGATTAGGAACAGTTCTCGGTTTAGGATTGCGGGAGGTAT
AG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

70.699

99.732

0.705