Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilL-C   Type   Machinery gene
Locus tag   RKE53_RS09975 Genome accession   NZ_CP134413
Coordinates   2234883..2237996 (+) Length   1037 a.a.
NCBI ID   WP_311131691.1    Uniprot ID   -
Organism   Microcystis aeruginosa NRERC-214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2229883..2242996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE53_RS09950 (RKE53_09950) - 2229888..2230256 (-) 369 WP_104395238.1 2Fe-2S iron-sulfur cluster-binding protein -
  RKE53_RS09955 (RKE53_09955) - 2230424..2232697 (-) 2274 WP_311131690.1 hypothetical protein -
  RKE53_RS09960 (RKE53_09960) - 2232694..2233635 (-) 942 WP_104395236.1 homogentisate phytyltransferase -
  RKE53_RS09970 (RKE53_09970) - 2234115..2234777 (-) 663 WP_288136952.1 class I SAM-dependent methyltransferase -
  RKE53_RS09975 (RKE53_09975) pilL-C 2234883..2237996 (+) 3114 WP_311131691.1 hybrid sensor histidine kinase/response regulator Machinery gene
  RKE53_RS09980 (RKE53_09980) - 2238149..2238898 (-) 750 WP_311131692.1 methyltransferase domain-containing protein -
  RKE53_RS09985 (RKE53_09985) - 2238980..2239471 (-) 492 WP_008197799.1 hypothetical protein -
  RKE53_RS09990 (RKE53_09990) uvrB 2239664..2241667 (+) 2004 WP_311131693.1 excinuclease ABC subunit UvrB -
  RKE53_RS09995 (RKE53_09995) - 2241672..2242679 (+) 1008 WP_311131694.1 flagellar assembly protein H -

Sequence

Protein


Download         Length: 1037 a.a.        Molecular weight: 113886.17 Da        Isoelectric Point: 4.3389

>NTDB_id=879986 RKE53_RS09975 WP_311131691.1 2234883..2237996(+) (pilL-C) [Microcystis aeruginosa NRERC-214]
MMKPDSDHFFNEDLEQLLESLEDKDTDGDETLDELSFLFEQTSPPAARYGASLDQTLSSLPLEPELAELFGDSINWEEAA
TNDLHSTRGTAPKSEPEDWDDLESLLLENAAVRDSEAIITISNPNFYDSLEDLEAFLEKPTPPEQSPLPDIFESLEVILW
ESTAGEPLAAAEPVQGLESLEIVPESSPEDEFKDLEKLLEETNQVVAATPAPSVSPPVGLSNLRPLVAKAFEQTTRVPIK
QLDNLSNLIGELVVKRNRLEQEQDRLRLFLDNLLNQVQNLSDVGGRMQDLYERSLLEGALLASRNAGGAIGYGGVQGKNQ
GNSTMTGELDALEIDRFTDFHLLSQEMIELIVRVRESASDIQFVVDETDQVTRSLRQATTQLQEGMTKSRMVPFSQTADH
LPRAIHDISLKLHKQAKLKVEGGDVLIDQMILENLNSPMIHLVNNAITHGIESPQERMAKGKPVHGTIWVRAFLQGNQTV
ITVSDDGAGIDVNLVKLKAIEKGLISDREAQNLSTQEVYEILFHPGFSTKDQADDFSGRGVGLDVVRISLIDVRGTVTID
SVLGKGSTFTLRLPLTLSICKALCCVSNHARIGFSMDGVEDMKDFRARDIQIDGEGRPCVFWQNTLLPFQPLSDLLSYNR
QLSRGSFYTGKQEEDSFSIVILRGGNNLLAVQVDQVIGEQEIVIKQIEGPIPKTAGIAGATVLGDGTVMPVGDVLELIDI
ARGRLRIDNGGLWHQSVPPVDVETSQKSEAMVLIVDDSITVRELLSLSFSKAGYRVEQARDGQEAWEKLRGGLPCDIVFC
DIEMPRMNGLELLSNLQKSPRLAAIPVALLTSRGSERHRQVAAKLGASGYFTKPYTERDLISAAERMIAGEVLLANSIKA
TSNQSLSSDTTIIDSNPANLLAQSSPLVLIVDDSLIVREMLMISLVKAGYRIEQARDGLEAWEKLQAGLACDLILCNIEM
PRLNGLELLSRLQEDEQLQGIPVAMITSGGTQKMQHLAAAKGAKGYFVKPWIEDVLLSAAQRLIAGEVLIQKENSVD

Nucleotide


Download         Length: 3114 bp        

>NTDB_id=879986 RKE53_RS09975 WP_311131691.1 2234883..2237996(+) (pilL-C) [Microcystis aeruginosa NRERC-214]
ATGATGAAACCGGACTCGGATCATTTCTTTAACGAAGATCTCGAACAGTTGTTAGAGAGTTTAGAAGACAAAGACACCGA
CGGTGACGAGACTCTAGACGAACTCAGCTTTTTATTTGAGCAGACTTCCCCGCCAGCGGCGAGGTATGGGGCTTCTCTTG
ACCAAACCCTGTCTTCCTTGCCCTTAGAACCGGAATTAGCAGAACTTTTTGGCGATAGCATCAATTGGGAAGAAGCCGCC
ACTAATGATCTTCATTCTACCCGTGGGACGGCTCCCAAGTCAGAACCAGAAGATTGGGATGATCTGGAATCGCTGCTGCT
AGAAAATGCAGCAGTCCGGGATTCAGAAGCAATTATAACCATCAGCAACCCGAATTTTTACGACAGCCTCGAAGACTTAG
AAGCTTTTCTGGAAAAACCCACTCCACCAGAACAATCGCCACTGCCGGACATCTTTGAATCCCTAGAGGTCATCCTCTGG
GAATCCACAGCAGGGGAGCCGCTGGCCGCGGCGGAACCTGTCCAAGGGTTAGAATCACTGGAAATTGTCCCAGAAAGCTC
CCCAGAGGATGAATTCAAGGATTTAGAAAAACTCCTCGAAGAAACCAATCAGGTGGTGGCAGCGACTCCGGCCCCCAGTG
TCAGTCCACCGGTTGGGTTAAGTAACCTGCGTCCCCTTGTTGCTAAAGCTTTTGAACAGACGACGCGGGTGCCGATCAAA
CAATTGGACAACCTCAGCAATCTGATCGGGGAACTGGTGGTTAAACGCAATCGCCTTGAACAGGAACAGGATCGCCTGCG
TCTCTTTTTAGATAATTTGCTTAACCAAGTCCAAAATCTCAGCGATGTGGGCGGTCGGATGCAGGATCTCTATGAAAGAA
GCCTCCTAGAAGGGGCTTTACTGGCTAGTCGCAATGCTGGTGGTGCCATCGGTTACGGTGGAGTTCAGGGAAAAAATCAG
GGCAATTCGACTATGACTGGGGAACTAGATGCCCTAGAGATCGACCGTTTTACCGATTTCCACTTATTATCCCAAGAGAT
GATCGAATTGATCGTGCGAGTGCGAGAATCGGCCTCTGATATTCAATTTGTCGTCGATGAAACCGATCAGGTGACGCGCA
GTCTGCGACAGGCTACCACTCAACTACAGGAAGGGATGACCAAATCGCGCATGGTTCCCTTTAGTCAAACTGCCGATCAT
CTACCCCGGGCAATTCACGATATTTCCCTGAAACTGCACAAACAAGCCAAATTAAAAGTGGAAGGGGGGGATGTTCTCAT
CGATCAGATGATCCTCGAAAATCTCAATAGTCCCATGATCCATCTGGTCAACAATGCGATCACCCACGGCATTGAATCAC
CCCAAGAACGCATGGCCAAAGGCAAACCTGTCCACGGTACGATCTGGGTCCGGGCATTCCTACAGGGCAATCAAACCGTG
ATCACCGTTAGTGATGATGGGGCTGGTATTGATGTTAATCTGGTTAAACTTAAGGCGATCGAAAAAGGCCTGATCAGCGA
TCGGGAAGCTCAAAACCTCAGTACCCAAGAAGTCTATGAAATTCTTTTTCATCCCGGTTTTAGTACCAAAGATCAGGCCG
ATGATTTTTCCGGCCGTGGGGTGGGTTTAGATGTGGTGCGGATCAGTTTAATCGATGTGCGCGGGACTGTGACTATTGAC
TCGGTACTGGGCAAAGGAAGCACTTTTACCCTCCGTTTACCCCTAACCCTGAGTATCTGTAAAGCCCTCTGTTGTGTTAG
TAATCATGCTCGCATTGGTTTCTCTATGGACGGAGTGGAAGATATGAAGGATTTCCGGGCCCGGGATATCCAAATAGATG
GGGAGGGACGGCCCTGCGTTTTCTGGCAAAACACTTTACTACCTTTCCAACCCCTGAGCGATTTGCTTTCCTACAATCGG
CAACTTAGTCGCGGTAGTTTTTACACCGGCAAACAGGAGGAAGACTCTTTTTCGATCGTAATCCTGCGCGGTGGTAATAA
TCTCCTCGCGGTACAGGTGGATCAAGTGATTGGGGAACAGGAGATCGTGATCAAACAGATCGAAGGACCGATTCCGAAAA
CGGCTGGGATTGCTGGGGCGACGGTTTTAGGGGATGGTACGGTGATGCCCGTCGGCGATGTGTTAGAGTTAATCGACATC
GCCAGGGGTCGTCTGCGTATCGATAACGGTGGTCTTTGGCACCAATCTGTACCGCCTGTAGATGTGGAAACTAGCCAAAA
ATCCGAGGCTATGGTTCTGATTGTCGATGATTCGATTACTGTTCGGGAATTGCTCTCTTTAAGCTTTAGTAAAGCTGGTT
ATCGTGTGGAACAGGCCCGGGATGGTCAAGAAGCTTGGGAAAAATTACGCGGTGGTTTGCCCTGTGATATCGTTTTCTGT
GATATCGAGATGCCTCGCATGAATGGCCTGGAATTACTGTCTAATTTGCAAAAATCCCCCCGATTAGCGGCGATTCCCGT
GGCTTTATTGACTTCCCGAGGCTCGGAACGTCATCGTCAGGTGGCGGCGAAATTAGGGGCTAGTGGTTATTTTACCAAGC
CCTACACGGAAAGAGATTTAATCTCGGCAGCTGAAAGAATGATTGCAGGGGAAGTGTTACTAGCTAATAGTATTAAAGCC
ACTTCTAATCAGTCTTTATCCTCCGATACAACAATTATTGATAGTAATCCCGCTAATTTGTTGGCACAATCGAGTCCTCT
CGTCTTGATTGTCGATGATTCGTTAATAGTGCGGGAAATGTTAATGATTTCTTTGGTTAAAGCTGGTTATCGCATTGAAC
AGGCCCGGGACGGTTTAGAAGCATGGGAAAAATTACAGGCCGGATTAGCTTGTGATCTGATTCTCTGCAATATCGAAATG
CCTCGCCTCAATGGGTTAGAATTGCTCTCTCGTCTGCAAGAGGATGAACAACTTCAGGGGATACCAGTGGCGATGATTAC
CTCGGGCGGGACGCAAAAAATGCAGCATCTCGCTGCTGCTAAAGGAGCAAAAGGTTATTTTGTCAAGCCCTGGATTGAGG
ATGTTTTACTGTCAGCAGCCCAACGTTTAATCGCTGGTGAGGTATTAATCCAAAAAGAAAATTCCGTGGATTAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(752-864)

Response regulator receiver domain (Response_reg)

(908-1019)

Signal transducing histidine kinase, homodimeric domain (H-kinase_dim)

(233-289)

CheW-like domain (CheW)

(587-719)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(439-576)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilL-C Synechocystis sp. PCC 6803

66.357

83.124

0.552