Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RJ610_RS05805 Genome accession   NZ_CP134270
Coordinates   1439602..1440768 (+) Length   388 a.a.
NCBI ID   WP_046655750.1    Uniprot ID   -
Organism   Lysobacter capsici strain XL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1434602..1445768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ610_RS05780 (RJ610_05780) - 1435424..1435897 (-) 474 WP_310980575.1 DUF4426 domain-containing protein -
  RJ610_RS05785 (RJ610_05785) proC 1435894..1436730 (-) 837 WP_310980576.1 pyrroline-5-carboxylate reductase -
  RJ610_RS05790 (RJ610_05790) - 1436940..1437464 (+) 525 WP_310980577.1 hypothetical protein -
  RJ610_RS05795 (RJ610_05795) - 1437582..1438283 (-) 702 WP_310980578.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJ610_RS05800 (RJ610_05800) pilT 1438459..1439496 (+) 1038 WP_036109068.1 type IV pilus twitching motility protein PilT Machinery gene
  RJ610_RS05805 (RJ610_05805) pilU 1439602..1440768 (+) 1167 WP_046655750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43133.57 Da        Isoelectric Point: 6.8286

>NTDB_id=879528 RJ610_RS05805 WP_046655750.1 1439602..1440768(+) (pilU) [Lysobacter capsici strain XL1]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITSGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEK
THECNFAIGVTGIGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIG
YRNLNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKDSVQLGMKTFDQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=879528 RJ610_RS05805 WP_046655750.1 1439602..1440768(+) (pilU) [Lysobacter capsici strain XL1]
ATGAATACCCCGGCCCCGACCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
TCAGCGCGCCTCGGACTTGTTCATCACCTCGGGCATGCCGCCGTCGATGAAGGTCCACGGCAAGATCAGCCCGATCACCC
AGAACCCGTTGACGCCGCAGCAAAGCCGCGACCTCGTGCTCAACGTGATGAACCCGCAGCAGCGCGAAGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGCATCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCGACCGTCGAAGAACTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCGACCGGCACCGGCAAGTCGACCTCGCTCGCGGCGATGATCGGC
TACCGCAATCTGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAATTCGTGCATCGCCACGAAGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGTCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGATCACGCCATCGCCTTCGCCGAAACCGGTCACCTGGTGCTG
TGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGTCGCAACCAGTT
GCTGATGGACTTGTCGCTTAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGCCGCG
TCGCCATGGAAATCCTGCTCGGCACCCCGCTGGCCCAGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAAGAGCTG
ATGAAGGACTCCGTGCAGCTCGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTA
CGAAGACGCCCTGCGCTACGCCGACTCGCAAAACGAAGTGCGCCTGCGCATCAAGCTGGCGCAAGGCGGCGATGCGCGGA
CTTTGTCGCAGGGGTTGGATGGGGTTGAGGTGGCTGAGGTCAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.755

94.588

0.66
  pilU Acinetobacter baylyi ADP1

63.866

92.01

0.588
  pilU Vibrio cholerae strain A1552

53.652

91.753

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

90.464

0.381