Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RHJ31_RS11005 Genome accession   NZ_CP134180
Coordinates   2237755..2239779 (+) Length   674 a.a.
NCBI ID   WP_302676616.1    Uniprot ID   -
Organism   Thermosynechococcus sp. JY1331     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2232755..2244779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHJ31_RS10975 (RHJ31_10975) leuD 2232971..2233561 (+) 591 WP_302676612.1 3-isopropylmalate dehydratase small subunit -
  RHJ31_RS10980 (RHJ31_10980) - 2233554..2234510 (-) 957 WP_302676613.1 glycosyltransferase family 2 protein -
  RHJ31_RS10985 (RHJ31_10985) - 2234601..2235563 (+) 963 WP_302676614.1 SPOR domain-containing protein -
  RHJ31_RS10990 (RHJ31_10990) - 2235560..2237035 (-) 1476 WP_310445807.1 right-handed parallel beta-helix repeat-containing protein -
  RHJ31_RS10995 (RHJ31_10995) - 2237083..2237280 (-) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHJ31_RS11000 (RHJ31_11000) - 2237281..2237493 (-) 213 WP_206201319.1 hypothetical protein -
  RHJ31_RS11005 (RHJ31_11005) pilB/pilB1 2237755..2239779 (+) 2025 WP_302676616.1 GspE/PulE family protein Machinery gene
  RHJ31_RS11010 (RHJ31_11010) pilT 2239847..2240944 (+) 1098 WP_206201317.1 type IV pilus twitching motility protein PilT Machinery gene
  RHJ31_RS11015 (RHJ31_11015) - 2240981..2242201 (+) 1221 WP_206201316.1 type II secretion system F family protein -
  RHJ31_RS11020 (RHJ31_11020) - 2242210..2242683 (+) 474 WP_206201315.1 J domain-containing protein -
  RHJ31_RS11025 (RHJ31_11025) - 2242680..2242952 (+) 273 WP_302676617.1 DUF3143 domain-containing protein -
  RHJ31_RS11030 (RHJ31_11030) - 2242949..2243302 (-) 354 WP_206201314.1 hypothetical protein -
  RHJ31_RS11035 (RHJ31_11035) - 2243852..2244656 (+) 805 Protein_2179 IS630 family transposase -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74546.91 Da        Isoelectric Point: 5.4345

>NTDB_id=879171 RHJ31_RS11005 WP_302676616.1 2237755..2239779(+) (pilB/pilB1) [Thermosynechococcus sp. JY1331]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDTDPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKNAPAEIGDINIEEDIEAMGSLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKATGQPICS
KCGGLGYKGRVGVYEIMRITERLQRLITEGAPTEQIKEAAVEEGMKTLLAYSLGLVKEGLTTLEEVERVTFTDTGLEAEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=879171 RHJ31_RS11005 WP_302676616.1 2237755..2239779(+) (pilB/pilB1) [Thermosynechococcus sp. JY1331]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGGGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACACTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACCGC
TAGCAAAAATGCCCCGGCTGAGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCAGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCTGAGGCGCTGAAAGGTGCAGAAGATGCGCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTTCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCCGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCTGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCAATTTTGCGAGCCTTTCTCCGCCAAGACCCCGATGTAATTCTGGTGGGCGAAACCCGTGACAAAGAAACCGCAAAAA
CCGCCATTGAAGCGGCTTTGACGGGGCACTTGGTCTTGACAACCCTGCACACTAACGATGCCCCCAGTGCAATTGCCCGC
CTCTCGGAAATGGGCATTGAACCCTTTATGGTTTCCGCCTCCCTCATTGGGGTTGTTGCCCAACGGCTGATGCGGCGGGT
ATGCAGTGAGTGTCGGATTCCCTATACACCCACCCGTGAGGAACTGGCGCGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTGACACCCGAGCAGATTCAAGCGGCTAAGGCAACTGGCCAACCCATTTGCAGT
AAATGTGGTGGTTTGGGCTACAAAGGACGGGTCGGCGTCTACGAAATTATGCGGATCACCGAGCGACTGCAACGGCTGAT
TACCGAAGGTGCCCCCACCGAGCAAATCAAGGAGGCGGCCGTCGAAGAGGGCATGAAAACCCTGCTTGCCTATAGCTTGG
GGTTAGTCAAGGAAGGACTCACCACCCTCGAAGAAGTGGAGCGCGTCACCTTTACGGACACTGGACTAGAGGCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.985

97.033

0.631
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.776

94.51

0.366