Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RHI63_RS04910 Genome accession   NZ_CP134175
Coordinates   1045709..1047733 (-) Length   674 a.a.
NCBI ID   WP_206201318.1    Uniprot ID   -
Organism   Thermosynechococcus sp. GLH187     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040709..1052733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHI63_RS04880 (RHI63_04880) - 1040954..1041636 (-) 683 Protein_954 IS630 transposase-related protein -
  RHI63_RS04885 (RHI63_04885) - 1042186..1042539 (+) 354 WP_206201314.1 hypothetical protein -
  RHI63_RS04890 (RHI63_04890) - 1042536..1042808 (-) 273 WP_149817855.1 DUF3143 domain-containing protein -
  RHI63_RS04895 (RHI63_04895) - 1042805..1043278 (-) 474 WP_310801582.1 J domain-containing protein -
  RHI63_RS04900 (RHI63_04900) - 1043287..1044507 (-) 1221 WP_206201316.1 type II secretion system F family protein -
  RHI63_RS04905 (RHI63_04905) pilT 1044544..1045641 (-) 1098 WP_206201317.1 type IV pilus twitching motility protein PilT Machinery gene
  RHI63_RS04910 (RHI63_04910) pilB/pilB1 1045709..1047733 (-) 2025 WP_206201318.1 GspE/PulE family protein Machinery gene
  RHI63_RS04915 (RHI63_04915) - 1047995..1048207 (+) 213 WP_206201319.1 hypothetical protein -
  RHI63_RS04920 (RHI63_04920) - 1048208..1048405 (+) 198 WP_206201320.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  RHI63_RS04925 (RHI63_04925) - 1048453..1049928 (+) 1476 WP_310743471.1 right-handed parallel beta-helix repeat-containing protein -
  RHI63_RS04930 (RHI63_04930) - 1049925..1050887 (-) 963 WP_206201322.1 SPOR domain-containing protein -
  RHI63_RS04935 (RHI63_04935) - 1050978..1051934 (+) 957 WP_206201323.1 glycosyltransferase family 2 protein -
  RHI63_RS04940 (RHI63_04940) leuD 1051927..1052517 (-) 591 WP_286626447.1 3-isopropylmalate dehydratase small subunit -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74401.80 Da        Isoelectric Point: 5.5500

>NTDB_id=879083 RHI63_RS04910 WP_206201318.1 1045709..1047733(-) (pilB/pilB1) [Thermosynechococcus sp. GLH187]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTASKSAPAAIGDINIEEDIEAMGGLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPSAIAR
LSEMGIEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKATGQPICS
KCGGLGYKGRVGVYEIMRITERLQRLITEGAPTEQIKEAAVEEGMKTLLAYSLGLVKEGLTTLEEVERVTFTDTGLEAEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=879083 RHI63_RS04910 WP_206201318.1 1045709..1047733(-) (pilB/pilB1) [Thermosynechococcus sp. GLH187]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGGGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACCGC
TAGCAAAAGCGCCCCGGCTGCGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCTGAGGCGCTGAAAGGTGCAGAAGATGCGCCGATTATTGCCCTTGTCAACAAAATT
TTGGCCAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTTCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCTGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCTGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCAATTTTGCGAGCCTTTCTCCGCCAAGACCCCGATGTAATTCTGGTGGGCGAAACCCGTGACAAAGAAACCGCAAAAA
CCGCCATTGAAGCGGCTTTGACGGGGCACTTGGTCTTGACAACCCTGCACACTAACGATGCCCCCAGTGCAATTGCCCGC
CTCTCGGAAATGGGCATTGAACCCTTTATGGTTTCCGCCTCCCTCATTGGGGTTGTTGCCCAACGGCTGATGCGGCGGGT
ATGCAGTGAGTGTCGGATTCCCTATACACCCACCCGTGAGGAACTGGCGCGCTTTGGCTTGTCTGCCTCCAAGGATGTCA
ACCTCACCCTCTACAAAGCCAATAAACTGACACCCGAGCAAATTCAAGCGGCTAAGGCAACTGGCCAACCCATTTGCAGT
AAATGTGGTGGTTTGGGCTACAAAGGACGGGTCGGCGTCTACGAAATTATGCGGATCACCGAGCGACTGCAACGGCTGAT
TACCGAAGGTGCCCCCACCGAGCAAATCAAGGAGGCGGCCGTCGAAGAGGGCATGAAAACCCTGCTTGCCTATAGCTTGG
GGTTAGTCAAGGAAGGACTCACCACCCTCGAAGAAGTGGAGCGCGTCACCTTTACGGACACTGGACTAGAGGCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.939

97.329

0.632
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.776

94.51

0.366