Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   RI108_RS20230 Genome accession   NZ_CP134081
Coordinates   4491312..4493531 (+) Length   739 a.a.
NCBI ID   WP_310791876.1    Uniprot ID   A0AAJ6MT79
Organism   Pseudomonas coleopterorum strain DJ13     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4486312..4498531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI108_RS20215 (RI108_20215) - 4488608..4489876 (-) 1269 WP_310791875.1 malic enzyme-like NAD(P)-binding protein -
  RI108_RS20220 (RI108_20220) - 4490067..4490837 (-) 771 WP_375143173.1 thermonuclease family protein -
  RI108_RS20225 (RI108_20225) rpmE 4490873..4491091 (-) 219 WP_043193699.1 50S ribosomal protein L31 -
  RI108_RS20230 (RI108_20230) priA 4491312..4493531 (+) 2220 WP_310791876.1 primosomal protein N' Machinery gene
  RI108_RS20235 (RI108_20235) argS 4493713..4495449 (+) 1737 WP_282348140.1 arginine--tRNA ligase -
  RI108_RS20240 (RI108_20240) - 4495451..4496140 (+) 690 WP_056844431.1 SPOR domain-containing protein -
  RI108_RS20245 (RI108_20245) hslV 4496412..4496942 (+) 531 WP_049861759.1 ATP-dependent protease subunit HslV -
  RI108_RS20250 (RI108_20250) hslU 4497017..4498354 (+) 1338 WP_043191381.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81620.05 Da        Isoelectric Point: 8.2615

>NTDB_id=878759 RI108_RS20230 WP_310791876.1 4491312..4493531(+) (priA) [Pseudomonas coleopterorum strain DJ13]
MPDVILRLALPSPLRRLFDYKAPAHTAQTVFTPGMRLRVPFGRREMIGILVEVVSHSEVPADKLKPAIALLDPVSPLPPA
LFKLCLWTSQYYQHGLGDTLSWALPVLLRQGEPAEARQERFWHAAADARLDDPRIARAPKQRDALKTLAQHPHGVAHQLL
SKLQLNKDSLDLLLAKQLVSVEVRRHGPGPRAEHWLAQPELPLNEEQRAAFEAIRASLGGFHAFLLAGVTGSGKTEVYLQ
LIHECLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRASQEDIPIVLGSATPSLESLHNAYTGRYGLLRIDHRAGGAQQPKFLRLDVKS
RPLDSGISGPMQQAIGQTLANGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARTTVHQRSSELRCHHCGHVERVPRQC
PKCGTVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILIGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALAERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDEACAVAEALVAQLGLGGLELLGPVPAPMERRAGRYRAQLLVQATLRAPLHRLLNAWMLQLEQ
LPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=878759 RI108_RS20230 WP_310791876.1 4491312..4493531(+) (priA) [Pseudomonas coleopterorum strain DJ13]
GTGCCCGACGTCATCCTGCGCCTCGCCCTGCCCTCGCCGCTGCGACGCCTGTTCGACTACAAGGCGCCCGCTCACACGGC
GCAGACCGTGTTCACCCCGGGCATGCGCCTGCGGGTACCGTTCGGGCGGCGGGAGATGATCGGCATACTGGTCGAGGTGG
TCAGCCACAGCGAGGTGCCGGCCGACAAACTCAAGCCGGCCATTGCCCTGCTCGATCCGGTCAGCCCGCTGCCGCCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTATCAGCACGGCCTGGGTGACACCCTCAGCTGGGCACTGCCGGTGCT
GCTGCGCCAGGGGGAACCGGCCGAGGCCCGCCAGGAGCGCTTCTGGCACGCCGCCGCCGATGCACGCCTGGACGACCCGC
GTATCGCCCGCGCGCCCAAGCAGCGCGACGCGCTCAAGACCCTGGCCCAGCATCCCCATGGCGTGGCGCATCAGCTGTTG
AGCAAGCTGCAGTTGAACAAGGACAGCCTCGACCTGCTGCTGGCCAAGCAACTGGTCAGCGTCGAAGTGCGTCGCCATGG
CCCCGGCCCACGCGCGGAACACTGGCTGGCGCAACCGGAACTGCCGCTCAACGAAGAGCAACGCGCCGCCTTCGAGGCGA
TCCGCGCCAGCCTGGGCGGCTTCCACGCGTTTCTCCTCGCGGGCGTGACCGGCAGTGGCAAGACCGAGGTGTACCTGCAA
CTGATTCACGAATGCCTGGAGGCCGGCAAGCAGGCCCTGGTGCTGATCCCGGAGATCAACCTGGGACCGCAGACCCTGGC
GCGCTTCGAACAGCGCTTCAACGCACGCATCGCGCTGGTGCATTCGGCGGTCAACGACCGCGACCGTCTCGACGCCTGGC
TGGCCGCCCGCGACGGCGAGGCCGACATCATCATCGGCACCCGCTCGGCGCTGTTCACGCCCATGAAGAACCCTGGGCTG
ATCATCATCGACGAAGAACACGATGGTTCCTATAAACAGCAGGAAGGCCTGCGCTACCACGCCCGTGACCTGGCGCTGGT
ACGCGCCAGCCAGGAAGACATTCCCATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GTCGCTACGGCCTGCTGCGCATCGACCATCGCGCCGGCGGCGCCCAGCAGCCCAAGTTCCTGCGCCTGGATGTGAAGAGC
CGACCGCTGGACAGCGGCATCAGCGGACCGATGCAGCAGGCCATTGGCCAGACCTTGGCCAACGGCCAGCAGGTGCTGGT
GTTTCTCAACCGCCGCGGCTTTGCCCCTACCCTGCTGTGCCACGATTGCGGCTGGCTGTCCGAGTGCCCGCGCTGCGATG
CGCGCACGACCGTTCACCAGCGCTCGAGCGAGCTGCGCTGCCATCACTGTGGCCACGTCGAACGCGTGCCGCGCCAGTGC
CCCAAGTGCGGCACGGTGGACCTGCGGCCGGTGGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGGCGATCCTGTTTCC
GGACTACCCGGTGCTGCGCGTCGACCGCGACAGCACCTCGCGCAAGGACGCGATGAACCAGCTGTTCGCGACCATCCAGA
AGGGCCAACCGTGCATCCTGATCGGCACCCAGATGCTGGCCAAGGGGCACCACTTTCCGCGGGTCACTCTGGTATCGATC
CTCGATGCCGACGGTGGGTTGTTCTCCGGTGACTTCCGCGCCAGCGAGCGCATGGCGCAATTGATCGTTCAGGTGGCCGG
CCGCGCCGGGCGTGCCGAAGAGCCAGGCAAGGTGATCATCCAGACGCATTTGGCCGACCATCCGCTGTTGGTGCAATTGA
CCGAGCAGGGTTACTTCGCCTTTGCCGAGCAGGCCCTGGCCGAACGCCGCGCCGCCGGCCTGCCACCCTTCGCGCACCTG
GCGCTGCTGCGCGCCGAGGCCCACAAGCCGGGCCAGGCCGAAGCCTTTCTGGACGAAGCCTGCGCCGTCGCCGAAGCCTT
GGTGGCGCAACTGGGACTGGGCGGCCTCGAACTGCTGGGGCCGGTGCCTGCCCCCATGGAACGCCGCGCCGGACGTTACC
GGGCACAGTTGCTGGTGCAGGCGACGCTGCGAGCACCCCTGCACCGCTTGCTCAATGCCTGGATGCTGCAACTGGAGCAA
CTGCCGAGCGGGCGCCAGGTGCGCTGGTCGCTGGATGTGGATCCGGTGGATTTGTATTGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(212-367)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48