Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RGB73_RS18280 Genome accession   NZ_CP134050
Coordinates   3686939..3687364 (+) Length   141 a.a.
NCBI ID   WP_396136116.1    Uniprot ID   -
Organism   Brevibacillus brevis strain NEB573     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3681939..3692364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB73_RS18270 (RGB73_18270) - 3683728..3685326 (-) 1599 WP_310764184.1 amidohydrolase -
  RGB73_RS18275 (RGB73_18275) - 3685515..3686804 (+) 1290 WP_310764187.1 MFS transporter -
  RGB73_RS18280 (RGB73_18280) nucA/comI 3686939..3687364 (+) 426 WP_396136116.1 sporulation protein Machinery gene
  RGB73_RS18285 (RGB73_18285) - 3687426..3688037 (-) 612 WP_310764189.1 TetR/AcrR family transcriptional regulator -
  RGB73_RS18290 (RGB73_18290) - 3688400..3688855 (+) 456 WP_310764191.1 MarR family transcriptional regulator -
  RGB73_RS18295 (RGB73_18295) - 3688852..3690258 (+) 1407 WP_310764193.1 MDR family MFS transporter -
  RGB73_RS18300 (RGB73_18300) lgt 3690300..3691091 (+) 792 WP_310764195.1 prolipoprotein diacylglyceryl transferase -
  RGB73_RS18305 (RGB73_18305) - 3691282..3691467 (+) 186 WP_310764197.1 hypothetical protein -
  RGB73_RS18310 (RGB73_18310) - 3691646..3692140 (-) 495 WP_310764198.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15720.88 Da        Isoelectric Point: 9.3525

>NTDB_id=878419 RGB73_RS18280 WP_396136116.1 3686939..3687364(+) (nucA/comI) [Brevibacillus brevis strain NEB573]
MQKKRILLIIAFLLALGSYFFERHSPQTQTSSIEADYVLEFPSSKYPQTAEHIRSAIAKGQSAVCTIDRRGADKNREKSL
KGVPTKPNYDRDEWPMAMCREGGSGADIAYISPADNRGAGAWIANRLEDYPDGTRVKIVVK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=878419 RGB73_RS18280 WP_396136116.1 3686939..3687364(+) (nucA/comI) [Brevibacillus brevis strain NEB573]
GTGCAGAAAAAACGAATCCTATTGATCATCGCCTTTTTGCTGGCGCTGGGCTCTTACTTTTTCGAGCGGCACAGCCCGCA
AACGCAGACTAGCAGCATCGAAGCGGATTACGTGCTGGAATTTCCGAGCAGCAAATACCCGCAAACAGCCGAGCACATCC
GCTCCGCGATCGCCAAGGGACAATCGGCGGTCTGTACCATCGACCGGAGGGGCGCAGACAAAAACCGTGAAAAATCACTC
AAGGGCGTCCCCACCAAGCCCAATTACGACCGCGACGAGTGGCCCATGGCGATGTGCCGGGAAGGCGGAAGCGGCGCGGA
TATCGCCTACATATCGCCCGCCGACAACCGCGGCGCCGGCGCGTGGATTGCCAACCGGCTCGAGGATTACCCGGATGGGA
CTCGGGTCAAAATCGTCGTCAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(44-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.016

86.525

0.511