Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RGB73_RS01270 Genome accession   NZ_CP134050
Coordinates   223106..224473 (+) Length   455 a.a.
NCBI ID   WP_310768044.1    Uniprot ID   -
Organism   Brevibacillus brevis strain NEB573     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 218106..229473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB73_RS01250 (RGB73_01250) - 218375..218839 (+) 465 WP_310768036.1 CtsR family transcriptional regulator -
  RGB73_RS01255 (RGB73_01255) - 218869..219387 (+) 519 WP_310768038.1 UvrB/UvrC motif-containing protein -
  RGB73_RS01260 (RGB73_01260) - 219406..220485 (+) 1080 WP_310768040.1 protein arginine kinase -
  RGB73_RS01265 (RGB73_01265) - 220550..223000 (+) 2451 WP_310768042.1 ATP-dependent Clp protease ATP-binding subunit -
  RGB73_RS01270 (RGB73_01270) radA 223106..224473 (+) 1368 WP_310768044.1 DNA repair protein RadA Machinery gene
  RGB73_RS01275 (RGB73_01275) disA 224483..225559 (+) 1077 WP_310768046.1 DNA integrity scanning diadenylate cyclase DisA -
  RGB73_RS01280 (RGB73_01280) pssA 225676..226404 (+) 729 WP_310768048.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  RGB73_RS01285 (RGB73_01285) - 226508..226903 (-) 396 WP_310768050.1 DUF1573 domain-containing protein -
  RGB73_RS01290 (RGB73_01290) - 227189..228277 (+) 1089 WP_310768052.1 PIN/TRAM domain-containing protein -
  RGB73_RS01295 (RGB73_01295) ispD 228300..228986 (+) 687 WP_310768053.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RGB73_RS01300 (RGB73_01300) ispF 228989..229462 (+) 474 WP_310768054.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49373.75 Da        Isoelectric Point: 6.6929

>NTDB_id=878400 RGB73_RS01270 WP_310768044.1 223106..224473(+) (radA) [Brevibacillus brevis strain NEB573]
MSKYKTKYACQECGYESPKWMGKCPGCNSWNTMVEEVTVKSAHRHEGMSGGQRQSAMPLTDVASEEEPRMDTTIAELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHQGAKVLYVSGEESAKQIKLRADRLGTKTPPMFVLAENDLDLIEQHI
NQIDPHVLIIDSIQTVFHPAVQSAAGSVAQVREATAQLMRIAKGKGIGTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVSNPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRMATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPDDIQVIGVSNIGEALNEVIRG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=878400 RGB73_RS01270 WP_310768044.1 223106..224473(+) (radA) [Brevibacillus brevis strain NEB573]
ATGTCAAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCAAAATGGATGGGCAAATGTCCGGGCTG
CAACAGCTGGAACACCATGGTCGAAGAGGTGACGGTGAAGTCGGCTCATCGCCATGAAGGGATGAGCGGAGGACAAAGGC
AGTCCGCGATGCCTTTGACTGATGTGGCCAGCGAGGAAGAGCCGAGGATGGACACGACCATCGCAGAATTGAATCGAGTT
CTGGGCGGCGGTCTGGTGCCCGGCTCGCTTATTTTAGTCGGCGGCGACCCGGGAATCGGAAAGTCGACGCTGCTGCTGCA
GACTTCCTTTGCTTTGGCGCATCAAGGAGCGAAAGTTCTGTATGTTTCCGGGGAGGAATCAGCAAAGCAGATCAAACTGC
GTGCGGACCGCCTCGGCACCAAGACGCCTCCGATGTTTGTTCTTGCAGAAAATGATTTGGATTTGATTGAGCAGCATATT
AATCAGATAGACCCTCATGTTTTGATTATCGACTCGATCCAGACCGTATTCCACCCCGCCGTCCAATCGGCTGCAGGCAG
CGTGGCCCAAGTCAGGGAGGCCACGGCACAGCTGATGAGGATCGCCAAAGGAAAGGGGATCGGCACCTTTATTGTGGGGC
ACGTTACCAAGGAAGGATCCATCGCCGGGCCGCGCATGCTCGAGCATATGGTGGATGCCGTCCTGTACTTTGAAGGGGAG
CGTCACAATACGTTCCGGATATTGCGCGCGGTCAAAAATCGATTTGGTTCGACAAACGAGATTGGCATATTTGAAATGAA
AGACCGCGGGCTGGAGGAAGTGTCCAATCCGTCCGAAATCTTTTTGGCGGAGCGTCCCTTTGGCGTGGCCGGATCGACGG
TAGTGGCCAGCATGGAAGGCACCCGTCCGGTCTTGGTCGAGCTGCAGGCGCTCGTGGCGCCGACGAGCTTCGTAACTCCG
CGCCGGATGGCGACAGGAGTGGATCACCAGCGGGTAGCGATGATCATGGCCGTTCTGGAAAAAAGAATGGGCATGATGCT
GCAAAACCAGGATGCTTACGTCAATGTGGCGGGAGGCGTTCGCCTGGATGAGCCAGCCGTAGATCTGGCCATCGCGGTGA
GCATTGCGAGCAGCTTTCGCGATCACGCGACCAATCCGCACGACGTGGTCATCGGCGAAATCGGATTGACCGGTGAAGTG
CGCGGGGTGTCCCGCATCGAACAGCGCGTCCGGGAAGCGCACAAGCTAGGCTTCAAGCGGGTGATCATCCCGGAAAAAAA
CATTCGCGGGCTGGACGCTCCGGACGATATCCAGGTAATCGGCGTATCCAATATCGGAGAGGCATTGAACGAGGTGATAA
GGGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(337-427)

AAA domain (AAA_25)

(70-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.662
  radA Streptococcus pneumoniae Rx1

58.499

99.56

0.582
  radA Streptococcus pneumoniae D39

58.499

99.56

0.582
  radA Streptococcus pneumoniae R6

58.499

99.56

0.582
  radA Streptococcus pneumoniae TIGR4

58.499

99.56

0.582
  radA Streptococcus mitis NCTC 12261

58.499

99.56

0.582
  radA Streptococcus mitis SK321

58.499

99.56

0.582