Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RGB74_RS07430 Genome accession   NZ_CP134049
Coordinates   1537708..1538457 (+) Length   249 a.a.
NCBI ID   WP_310762350.1    Uniprot ID   -
Organism   Bacillus sp. NEB1478     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1532708..1543457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB74_RS07400 (RGB74_07400) - 1533141..1535261 (+) 2121 WP_310762345.1 HD family phosphohydrolase -
  RGB74_RS07405 (RGB74_07405) ybeY 1535291..1535728 (+) 438 WP_396136054.1 rRNA maturation RNase YbeY -
  RGB74_RS07410 (RGB74_07410) - 1535725..1536093 (+) 369 WP_396136029.1 diacylglycerol kinase family protein -
  RGB74_RS07415 (RGB74_07415) - 1536126..1536524 (+) 399 WP_310762347.1 cytidine deaminase -
  RGB74_RS07420 (RGB74_07420) era 1536517..1537425 (+) 909 WP_310762348.1 GTPase Era -
  RGB74_RS07425 (RGB74_07425) - 1537524..1537667 (+) 144 WP_310762349.1 YqzL family protein -
  RGB74_RS07430 (RGB74_07430) recO 1537708..1538457 (+) 750 WP_310762350.1 DNA repair protein RecO Machinery gene
  RGB74_RS07435 (RGB74_07435) - 1538556..1539200 (+) 645 WP_396136055.1 helix-turn-helix transcriptional regulator -
  RGB74_RS07440 (RGB74_07440) - 1539218..1540033 (+) 816 WP_310762352.1 pyruvate, water dikinase regulatory protein -
  RGB74_RS07445 (RGB74_07445) - 1540413..1540901 (+) 489 WP_310762353.1 DUF188 domain-containing protein -
  RGB74_RS07450 (RGB74_07450) dnaG 1540962..1542770 (+) 1809 WP_310762354.1 DNA primase -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28531.24 Da        Isoelectric Point: 9.3344

>NTDB_id=878371 RGB74_RS07430 WP_310762350.1 1537708..1538457(+) (recO) [Bacillus sp. NEB1478]
MLYKVEGIVIKTIPYGESNTIITLYTKELGKIGVMARGAKKPKSRFTSITQLFTYGIFVFQRTNKGLGTLQQGEALSSFR
RIREDLVKTAYAAYLSELLDRLTTENDLKPDLFGWIKQALEYIDHGIDPEVITFLFEIKMMKTAGIVPELSKCVSCGKME
GNFSFSIREGGFLCERCAYKDPYRMNISSSAVRLLHMFYDFDLARLGNVTLKKETKRELRTLFDAYMDEYSGVFLKSKKF
LKQLDQLSD

Nucleotide


Download         Length: 750 bp        

>NTDB_id=878371 RGB74_RS07430 WP_310762350.1 1537708..1538457(+) (recO) [Bacillus sp. NEB1478]
ATGCTTTATAAAGTAGAAGGAATAGTCATAAAAACCATTCCGTATGGTGAATCAAATACGATTATTACACTGTACACAAA
AGAATTGGGAAAAATAGGCGTAATGGCAAGAGGGGCGAAAAAACCAAAAAGCCGCTTCACTTCCATTACGCAATTATTTA
CATATGGTATATTTGTTTTTCAAAGAACGAATAAAGGTTTAGGTACACTGCAGCAAGGAGAAGCTCTCTCGTCTTTTAGA
AGGATACGTGAAGACCTCGTAAAAACAGCATATGCTGCCTATTTATCTGAACTGCTTGACCGGTTAACAACAGAAAATGA
CTTGAAACCAGATCTATTTGGCTGGATAAAGCAAGCCTTGGAATATATTGACCATGGTATTGATCCAGAGGTCATAACCT
TTTTATTTGAAATCAAAATGATGAAAACAGCTGGAATCGTTCCGGAACTTAGCAAGTGCGTATCTTGCGGTAAAATGGAA
GGTAACTTTTCGTTCTCGATAAGAGAAGGAGGTTTTCTTTGTGAAAGATGCGCATACAAGGATCCATACAGGATGAATAT
TTCTTCAAGCGCTGTCCGTTTGCTGCACATGTTTTATGATTTTGATCTGGCAAGACTCGGAAATGTTACTTTAAAAAAAG
AAACAAAACGTGAATTGAGGACTTTGTTTGATGCCTATATGGATGAATATTCAGGTGTCTTTCTAAAATCTAAGAAATTT
TTAAAACAACTTGATCAATTATCCGATTAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-79)

Recombination protein O C terminal (RecO_C)

(83-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.197

0.55