Detailed information NTG0878067

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RI210_RS00790
• Genome accession: NZ_CP133985
• Coordinates: 166481..167635 (-)
• Length: 384 a.a.
• NCBI ID: WP_310751496.1
• Uniprot ID: -
• Organism: Methylomonas koyamae strain MY1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 161481..172635

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RI210_RS00765 (RI210_00765)	-	162004..162726 (+)	723	WP_054759463.1	cytochrome c1	-
RI210_RS00770 (RI210_00770)	ftsH	162812..164725 (-)	1914	WP_310751493.1	ATP-dependent zinc metalloprotease FtsH	-
RI210_RS00775 (RI210_00775)	lexA	165298..165927 (+)	630	WP_064023307.1	transcriptional repressor LexA	-
RI210_RS00780 (RI210_00780)	-	165940..166089 (+)	150	WP_157198033.1	hypothetical protein	-
RI210_RS00785 (RI210_00785)	-	166135..166458 (+)	324	WP_064040827.1	hypothetical protein	-
RI210_RS00790 (RI210_00790)	pilU	166481..167635 (-)	1155	WP_310751496.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RI210_RS00795 (RI210_00795)	-	167797..170406 (+)	2610	WP_310751500.1	PAS domain-containing protein	-
RI210_RS00800 (RI210_00800)	-	170403..171035 (+)	633	WP_064020087.1	response regulator transcription factor	-
RI210_RS00805 (RI210_00805)	-	171203..172246 (+)	1044	WP_310751503.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
RI210_RS00810 (RI210_00810)	-	172243..172467 (+)	225	WP_310751506.1	sulfurtransferase TusA family protein	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 42469.60 Da
• Isoelectric Point: 6.0981

>NTDB_id=878067 RI210_RS00790 WP_310751496.1 166481..167635(-) (pilU) [Methylomonas koyamae strain MY1]
MEFRDYLKILVQHDGSDLYLTAGAPPAAKFQGTLKPLENVKLTPERIRQIADSVMDNEQRASFEHVPEMNLAITEEGVGR
FRVNIFKQRNCYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKRSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFVSQRLVPTIEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENVGMQTFDSHL
LKLYKSGVISLEEAMRNSDSPNNLKLKINLSEGLGSATKPAGALGSLALEEISKKEGEDGDEGH

Nucleotide

• Length: 1155 bp

>NTDB_id=878067 RI210_RS00790 WP_310751496.1 166481..167635(-) (pilU) [Methylomonas koyamae strain MY1]
ATGGAATTTAGAGACTATTTGAAAATCTTGGTGCAGCACGACGGTTCCGATTTGTATTTGACGGCCGGTGCGCCTCCGGC
TGCAAAATTTCAAGGCACCTTGAAGCCGCTGGAAAACGTCAAGCTGACTCCGGAACGGATCAGACAGATTGCCGACAGCG
TGATGGACAACGAGCAGCGCGCCAGTTTCGAGCACGTACCGGAGATGAATCTGGCGATTACCGAAGAGGGCGTCGGCCGA
TTCCGGGTCAATATTTTCAAGCAACGCAATTGTTATGCGTTGGTGATCCGCAATATCAAGGTGGATATCCCTAACGCCGA
CGTTTTAGGCTTGCCGCAAATTTTGAAAGACAAAATCATGGAAAAGCGCGGCCTGATTTTGTTCGTCGGCGGCACTGGTT
CCGGCAAATCAACCTCGCTGGCAGCCTTGATCGATTACCGCAACAGCAATTCGTCGGGCCACATCATCACCATCGAAGAC
CCGATCGAATTCGTTCACCCGCATAAGCGTTCGCTGGTCAACCAGCGCGAAGTTGGCGTGGACACGCTGAGTTACGAGGA
CGCATTGAAGAACACCTTACGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACGATGGAACATG
CGTTAGCCTTTGCCGAAACCGGACATTTGTGCCTGTCGACCTTGCATGCCAATAACGCCAACCAGGCGCTGGATAGGATC
ATCAATTTCTTCCCGGAAGAACGCCGGCCGCAATTGTTGATGGACTTGTCGTTGAATTTGCAGGCCTTCGTGTCGCAACG
CCTGGTACCGACCATCGAAGGCAAGCGGGTTGCGGCAATCGAAATTCTGTTGGGCACTAAATTGGTCAGCGATTTGATCC
ATAAGGGCGACATTCACGCGATCAAGGAGGCGATGGAAAAGTCGGAAAACGTCGGTATGCAGACCTTCGACAGCCATCTA
TTGAAGCTGTACAAAAGCGGCGTGATCTCACTGGAAGAAGCGATGCGCAATTCCGATTCGCCGAACAATCTGAAATTGAA
AATCAACTTGAGCGAGGGCTTGGGTTCGGCGACCAAACCGGCCGGGGCGCTGGGCAGTCTGGCTTTGGAAGAAATCAGCA
AGAAGGAAGGCGAGGACGGCGACGAAGGCCATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.835	91.406	0.529
pilU	Acinetobacter baylyi ADP1	54.696	94.271	0.516
pilU	Vibrio cholerae strain A1552	49.435	92.188	0.456
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.052	100	0.404
pilT	Legionella pneumophila strain Lp02	42.424	85.938	0.365
pilT	Legionella pneumophila strain ERS1305867	42.424	85.938	0.365