Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RHO14_RS12615 Genome accession   NZ_CP133967
Coordinates   2833243..2834673 (+) Length   476 a.a.
NCBI ID   WP_392553645.1    Uniprot ID   -
Organism   Orbus wheelerorum strain lpD04     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2828243..2839673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHO14_RS12590 (RHO14_12465) - 2829660..2830841 (-) 1182 WP_392552241.1 efflux RND transporter periplasmic adaptor subunit -
  RHO14_RS12595 (RHO14_12470) yccX 2831000..2831302 (+) 303 WP_392552242.1 acylphosphatase -
  RHO14_RS12600 (RHO14_12475) lptC 2831374..2831955 (+) 582 WP_392552243.1 LPS export ABC transporter periplasmic protein LptC -
  RHO14_RS12605 (RHO14_12480) lptA 2831968..2832516 (+) 549 WP_392552244.1 lipopolysaccharide transport periplasmic protein LptA -
  RHO14_RS12610 (RHO14_12485) lptB 2832518..2833243 (+) 726 WP_392552245.1 LPS export ABC transporter ATP-binding protein -
  RHO14_RS12615 (RHO14_12490) rpoN/rpoN1 2833243..2834673 (+) 1431 WP_392553645.1 RNA polymerase factor sigma-54 Machinery gene
  RHO14_RS12620 (RHO14_12495) rapZ 2834715..2835575 (+) 861 WP_392552246.1 RNase adapter RapZ -
  RHO14_RS12625 (RHO14_12500) - 2835692..2836027 (-) 336 WP_392552247.1 NINE protein -
  RHO14_RS12630 (RHO14_12505) - 2836110..2836931 (-) 822 WP_392552248.1 divergent polysaccharide deacetylase family protein -
  RHO14_RS12635 (RHO14_12510) envC 2836928..2838142 (-) 1215 WP_392552249.1 murein hydrolase activator EnvC -

Sequence

Protein


Download         Length: 476 a.a.        Molecular weight: 54409.80 Da        Isoelectric Point: 4.7339

>NTDB_id=878064 RHO14_RS12615 WP_392553645.1 2833243..2834673(+) (rpoN/rpoN1) [Orbus wheelerorum strain lpD04]
MKQSLQLKISQQLAMTPQLQQAIRLLQLSTQELCQEIQVLLENNPLLEIDDNFNEISIESSPESDNIDTSEMMENREISI
DISLDASLDDIYSAGTPSGTFSDYHNDELPIYQGETHQTLNEYLMWQLNLTPFSERDKAIAISLIQSIDEKGYLSCSIQD
ILEEQGNDEVELDEVEAVLKRIQHFDPVGIGARTLQECLTLQVNQLYPITNELRYAKIIIDNYLELLGQKDFKSLQKKLS
IDQESLKHTINLIQQLHPYPGDLINTSAPEYIIPDVFVKKIAEKWLVLINNDAIPRLRINQHYAAMEKITNENDSKYIHQ
ALQDANWLIKSIENRNDTLTKVSRFIVKHQQGFLDHGEEYMKPMILADVANAINMHESTISRITASKYLQCPRGIFELKY
FFSSHVNTESGGEASSTAIRALIKKWISQESCQKPLSDNKIANLLEEQGIIVARRTIAKYRELLSIPSSSQRKTLK

Nucleotide


Download         Length: 1431 bp        

>NTDB_id=878064 RHO14_RS12615 WP_392553645.1 2833243..2834673(+) (rpoN/rpoN1) [Orbus wheelerorum strain lpD04]
ATAAAACAGAGTCTACAATTAAAAATTTCACAGCAGTTGGCTATGACGCCACAATTGCAACAAGCAATTCGCCTGTTGCA
ACTATCTACTCAAGAATTATGCCAAGAAATTCAAGTCTTACTAGAAAATAATCCTTTACTTGAAATCGATGATAATTTCA
ATGAAATCAGCATTGAGAGTAGTCCTGAATCAGATAATATTGATACATCAGAAATGATGGAAAATCGGGAAATTTCAATT
GATATATCACTTGATGCATCTCTCGATGATATTTATAGCGCTGGCACACCGTCAGGCACTTTTTCCGACTATCACAATGA
TGAGCTGCCTATCTATCAAGGAGAAACACATCAAACGTTAAATGAGTATTTAATGTGGCAATTAAATTTAACACCGTTTA
GCGAACGAGATAAAGCTATCGCCATTTCATTGATCCAATCAATTGATGAGAAAGGATATTTAAGCTGCTCAATACAAGAT
ATTTTAGAAGAACAAGGTAATGATGAAGTCGAACTTGATGAAGTCGAAGCGGTACTCAAACGAATTCAGCACTTTGACCC
AGTTGGTATCGGAGCAAGAACATTGCAAGAGTGCCTAACTTTGCAAGTGAATCAACTTTACCCGATAACTAACGAATTAA
GATATGCAAAAATAATTATTGATAATTATTTAGAATTACTTGGTCAAAAAGACTTCAAATCACTACAGAAAAAGCTAAGT
ATCGACCAAGAGTCACTCAAACATACAATTAATTTAATTCAACAACTACATCCCTATCCAGGTGACTTGATTAACACATC
CGCTCCTGAGTATATTATTCCAGATGTATTTGTAAAAAAAATAGCAGAAAAATGGTTAGTACTTATTAATAATGACGCTA
TCCCTCGTTTAAGAATCAATCAGCACTACGCCGCAATGGAAAAAATAACTAATGAAAACGATAGCAAATATATCCATCAA
GCATTACAAGATGCCAATTGGTTAATCAAAAGCATTGAAAACCGTAACGATACACTTACAAAAGTGAGTAGATTCATCGT
AAAGCATCAGCAAGGTTTTCTTGATCATGGCGAGGAATATATGAAGCCAATGATCTTAGCCGACGTTGCCAATGCGATTA
ATATGCATGAGTCAACCATTTCTCGAATTACTGCCTCAAAATATTTGCAATGCCCTCGTGGAATTTTTGAGTTAAAATAT
TTTTTTTCGAGCCATGTTAATACAGAATCAGGAGGTGAGGCATCCTCTACAGCAATAAGAGCTTTAATTAAAAAGTGGAT
CAGTCAAGAAAGTTGCCAAAAGCCACTAAGCGATAATAAAATAGCAAACTTACTTGAAGAGCAAGGAATTATTGTTGCAA
GGCGAACAATTGCAAAGTATAGAGAACTATTATCAATTCCATCATCAAGCCAACGAAAAACACTAAAATAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(316-473)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.365

100

0.483