Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RHN62_RS06500 Genome accession   NZ_CP133937
Coordinates   1363724..1364158 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain AKS2021-HT3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1358724..1369158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHN62_RS06470 - 1359285..1359554 (-) 270 WP_241951066.1 GNAT family N-acetyltransferase -
  RHN62_RS06475 yacG 1359554..1359757 (-) 204 WP_241951067.1 DNA gyrase inhibitor YacG -
  RHN62_RS06480 coaE 1359768..1360388 (-) 621 WP_241951068.1 dephospho-CoA kinase -
  RHN62_RS06485 - 1360403..1361125 (-) 723 WP_241951069.1 A24 family peptidase -
  RHN62_RS06490 - 1361125..1362336 (-) 1212 WP_241952331.1 type II secretion system F family protein -
  RHN62_RS06495 pilB 1362336..1363724 (-) 1389 WP_241951070.1 GspE/PulE family protein Machinery gene
  RHN62_RS06500 pilA 1363724..1364158 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  RHN62_RS06505 ampD 1364318..1364884 (+) 567 WP_241951071.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHN62_RS06510 rppH 1365470..1366066 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RHN62_RS06515 - 1366069..1366863 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RHN62_RS06520 lgt 1366873..1367676 (+) 804 WP_241951072.1 prolipoprotein diacylglyceryl transferase -
  RHN62_RS06525 - 1367682..1368533 (+) 852 WP_115097855.1 thymidylate synthase -
  RHN62_RS06530 tadA 1368560..1369048 (+) 489 WP_241951073.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=877577 RHN62_RS06500 WP_005741096.1 1363724..1364158(-) (pilA) [Pasteurella multocida strain AKS2021-HT3]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=877577 RHN62_RS06500 WP_005741096.1 1363724..1364158(-) (pilA) [Pasteurella multocida strain AKS2021-HT3]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
TTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368