Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RHN82_RS12225 Genome accession   NZ_CP133860
Coordinates   2562849..2564282 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. EB5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557849..2569282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHN82_RS12195 (RHN82_12195) elbB 2559381..2560034 (+) 654 WP_047051241.1 isoprenoid biosynthesis glyoxalase ElbB -
  RHN82_RS12200 (RHN82_12200) mtgA 2560031..2560756 (+) 726 WP_047051240.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RHN82_RS12205 (RHN82_12205) npr 2560796..2561068 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  RHN82_RS12210 (RHN82_12210) rapZ 2561065..2561919 (-) 855 WP_003861870.1 RNase adapter RapZ -
  RHN82_RS12215 (RHN82_12215) ptsN 2561965..2562456 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RHN82_RS12220 (RHN82_12220) hpf 2562539..2562826 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  RHN82_RS12225 (RHN82_12225) rpoN/rpoN1 2562849..2564282 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  RHN82_RS12230 (RHN82_12230) lptB 2564330..2565055 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  RHN82_RS12235 (RHN82_12235) lptA 2565062..2565616 (-) 555 WP_015571984.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RHN82_RS12240 (RHN82_12240) lptC 2565585..2566160 (-) 576 WP_047051239.1 LPS export ABC transporter periplasmic protein LptC -
  RHN82_RS12245 (RHN82_12245) kdsC 2566157..2566723 (-) 567 WP_047051238.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RHN82_RS12250 (RHN82_12250) kdsD 2566738..2567724 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  RHN82_RS12255 (RHN82_12255) - 2567738..2568715 (-) 978 WP_047051237.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=877262 RHN82_RS12225 WP_003861864.1 2562849..2564282(-) (rpoN/rpoN1) [Enterobacter cloacae complex sp. EB5]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=877262 RHN82_RS12225 WP_003861864.1 2562849..2564282(-) (rpoN/rpoN1) [Enterobacter cloacae complex sp. EB5]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGTAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTAGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
AATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCCCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATATATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48