Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RHM62_RS00430 Genome accession   NZ_CP133777
Coordinates   84101..85582 (+) Length   493 a.a.
NCBI ID   WP_322123647.1    Uniprot ID   -
Organism   Actimicrobium sp. CCC2.4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 79101..90582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM62_RS00405 (RHM62_00405) - 80529..81578 (+) 1050 WP_322123642.1 KpsF/GutQ family sugar-phosphate isomerase -
  RHM62_RS00410 (RHM62_00410) - 81578..82114 (+) 537 WP_322123643.1 HAD family hydrolase -
  RHM62_RS00415 (RHM62_00415) lptC 82111..82707 (+) 597 WP_322123644.1 LPS export ABC transporter periplasmic protein LptC -
  RHM62_RS00420 (RHM62_00420) lptA 82752..83306 (+) 555 WP_322123645.1 lipopolysaccharide transport periplasmic protein LptA -
  RHM62_RS00425 (RHM62_00425) lptB 83315..84088 (+) 774 WP_322123646.1 LPS export ABC transporter ATP-binding protein -
  RHM62_RS00430 (RHM62_00430) rpoN/rpoN1 84101..85582 (+) 1482 WP_322123647.1 RNA polymerase factor sigma-54 Machinery gene
  RHM62_RS00435 (RHM62_00435) hpf 85690..86046 (+) 357 WP_322123648.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RHM62_RS00440 (RHM62_00440) - 86148..87245 (+) 1098 WP_322123649.1 enoyl-CoA hydratase/isomerase family protein -
  RHM62_RS00445 (RHM62_00445) - 87273..88403 (-) 1131 WP_322123650.1 deoxyguanosinetriphosphate triphosphohydrolase -
  RHM62_RS00450 (RHM62_00450) aroKB 88423..90093 (-) 1671 WP_322125282.1 bifunctional shikimate kinase/3-dehydroquinate synthase AroKB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54930.61 Da        Isoelectric Point: 5.1057

>NTDB_id=876639 RHM62_RS00430 WP_322123647.1 84101..85582(+) (rpoN/rpoN1) [Actimicrobium sp. CCC2.4]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQMLTDNPLLERLDDPLDHSVRLLADGALGAEGIRQEGASEDA
NAAPSSSESEAPAPDQNDAGDATPSTDADWSFDDVARTSKAPEDDDARPQLEAHESTLREFLLEQMRVTVRNPRDRALVE
LMIDALNDNGYLDDTLEAMLERLPAELDVEAEELTIALKLLQSFDPPGVGARHAAECLALQIRRFPGIAMVTRRMALVIV
EDYLPLFAQRDFSKLKKALDCDDEDLREAQSVIRQCNPHPGAIFGGGTSDYVVPDVIVKRTKTGWQVMLNHDVMPRLRVN
AMYANILKQSKGEGSLTSQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTISR
VTTQKYMLTPHGMFELKYFFGSHVATETGGEASSTAIRALIKQLIGAEDPKIPFSDSKIADMLGEQGMVVARRTVAKYRE
ALKIPPVNLRKAL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=876639 RHM62_RS00430 WP_322123647.1 84101..85582(+) (rpoN/rpoN1) [Actimicrobium sp. CCC2.4]
ATGAAACAATCCCTCCAGCTCCGCACCTCCCAGCATCTTGCGCTGACGCCGCAATTGCAGCAATCGATCCGCCTGTTGCA
ACTGTCGACGCTGGAACTGCATCAGGAACTGGAGCAAATGCTGACCGATAATCCGCTGCTGGAACGGCTCGACGATCCGC
TCGATCACTCGGTCCGTCTGCTGGCGGATGGCGCGCTCGGAGCCGAAGGTATCCGTCAGGAAGGGGCCAGCGAAGACGCC
AACGCAGCGCCTTCATCGTCCGAATCGGAAGCCCCCGCGCCGGACCAGAACGATGCCGGCGACGCCACGCCAAGCACCGA
TGCGGACTGGAGTTTTGATGACGTTGCACGCACCTCGAAAGCGCCGGAAGACGACGATGCCAGGCCGCAGCTCGAAGCAC
ACGAATCCACGCTGCGCGAATTCCTGCTCGAGCAAATGCGCGTCACGGTGCGCAACCCGCGCGATCGCGCGCTGGTCGAA
TTGATGATCGATGCGCTCAATGACAATGGCTACCTCGACGATACGCTGGAGGCCATGCTCGAGCGCTTGCCGGCCGAACT
CGACGTTGAAGCCGAAGAGCTGACCATCGCGCTCAAACTGCTGCAGAGCTTCGATCCGCCCGGCGTCGGCGCCCGCCACG
CGGCCGAGTGCCTGGCGCTGCAGATACGGCGTTTTCCCGGCATCGCGATGGTGACCCGGCGCATGGCACTGGTTATCGTC
GAGGACTATCTGCCGCTGTTTGCACAGCGCGATTTCAGCAAGCTCAAGAAAGCCCTCGACTGCGATGACGAGGATCTGCG
CGAAGCCCAGAGCGTGATCCGCCAGTGCAATCCGCACCCGGGTGCGATCTTCGGCGGCGGCACCTCGGACTACGTGGTGC
CGGACGTGATCGTCAAGCGCACCAAGACCGGCTGGCAAGTCATGCTCAACCATGACGTGATGCCACGACTGCGGGTCAAT
GCCATGTACGCCAACATTCTCAAGCAGAGCAAAGGTGAGGGATCGCTGACGTCGCAGCTGCAGGAAGCCAAGTGGCTGAT
CAAAAATATGCGCCAGCGATTCGATACGATCCTGCGGGTTGCACAGGCCATCGTCGAGCGTCAGCGGAACTTTTTTTCCC
ATGGCGCGGTCGCCATGCGCCCCCTTGTCTTGCGTGAGATTGCTGATACACTCGGACTGCACGAGAGCACCATCTCACGT
GTGACAACCCAGAAATACATGCTGACCCCGCATGGCATGTTCGAGTTGAAATATTTTTTTGGCAGCCACGTTGCCACCGA
AACGGGCGGCGAAGCGTCATCGACGGCAATACGGGCACTCATCAAACAATTGATAGGAGCTGAAGACCCGAAGATTCCAT
TTTCTGACAGTAAGATCGCAGACATGCTGGGAGAACAGGGTATGGTAGTGGCAAGGCGTACGGTCGCAAAATATCGCGAA
GCCCTGAAAATCCCTCCCGTCAACCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.196

100

0.623