Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RHM64_RS20720 Genome accession   NZ_CP133776
Coordinates   4593994..4595361 (-) Length   455 a.a.
NCBI ID   WP_322166149.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4588994..4600361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS20700 (RHM64_20700) yjiA 4589960..4590940 (-) 981 WP_322166145.1 GTPase -
  RHM64_RS20705 (RHM64_20705) - 4591015..4591212 (-) 198 WP_322166146.1 YbdD/YjiX family protein -
  RHM64_RS20710 (RHM64_20710) - 4591205..4593310 (-) 2106 WP_322166147.1 carbon starvation CstA family protein -
  RHM64_RS20715 (RHM64_20715) - 4593517..4593885 (+) 369 WP_322166148.1 PilZ domain-containing protein -
  RHM64_RS20720 (RHM64_20720) radA 4593994..4595361 (-) 1368 WP_322166149.1 DNA repair protein RadA Machinery gene
  RHM64_RS20725 (RHM64_20725) mscL 4595556..4596005 (+) 450 WP_322166150.1 large-conductance mechanosensitive channel protein MscL -
  RHM64_RS20730 (RHM64_20730) - 4596059..4596835 (-) 777 WP_322166151.1 ferredoxin--NADP reductase -
  RHM64_RS20735 (RHM64_20735) - 4597032..4597499 (+) 468 WP_322166152.1 helix-turn-helix transcriptional regulator -
  RHM64_RS20740 (RHM64_20740) - 4597645..4598769 (+) 1125 WP_322166153.1 methyltransferase -
  RHM64_RS20745 (RHM64_20745) - 4598938..4599105 (-) 168 WP_322166154.1 DUF2474 domain-containing protein -
  RHM64_RS20750 (RHM64_20750) cydB 4599119..4600126 (-) 1008 WP_322166155.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48902.41 Da        Isoelectric Point: 6.5051

>NTDB_id=876630 RHM64_RS20720 WP_322166149.1 4593994..4595361(-) (radA) [Pseudomonas sp. AH2]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLVETMLENGAATPPSGRTGWTGQKAQIRTLAEVSVEEMPRFTTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCALAEQMPALYVTGEESQQQVAMRARRLGLAQDKLRVMTETCIETIIA
TARIEQPKVMVIDSIQTIYTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAEEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVISSLRNRPLPHDLLVFGEIGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=876630 RHM64_RS20720 WP_322166149.1 4593994..4595361(-) (radA) [Pseudomonas sp. AH2]
ATGGCCAAGGCTAAGCGCATGTATGGCTGCACGGAGTGCGGGGCGACTTTTCCGAAATGGGCTGGCCAGTGCGGCGAATG
CGGCGCTTGGAACACCTTGGTCGAAACGATGCTTGAAAATGGTGCCGCCACGCCACCCAGCGGACGTACCGGCTGGACTG
GGCAAAAAGCACAGATCAGAACCTTGGCCGAAGTCAGCGTCGAGGAGATGCCGCGATTTACCACTAACTCCACCGAACTC
GACCGGGTACTCGGCGGCGGTTTGGTGGACGGCTCGGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCCACCAT
TCTGTTGCAAACCCTTTGCGCGCTGGCTGAGCAAATGCCCGCGCTGTACGTCACCGGCGAAGAATCGCAGCAGCAAGTTG
CCATGCGCGCTCGTCGTTTGGGGCTGGCGCAAGACAAACTTCGGGTCATGACCGAAACCTGTATCGAAACCATTATTGCC
ACGGCGCGCATCGAACAGCCCAAGGTCATGGTCATCGACTCGATCCAGACCATTTACACCGAACAATTGCAGTCCGCGCC
GGGCGGCGTGTCACAAGTGCGTGAAAGCGCGGCGTTGCTGGTGCGCTACGCCAAACAAAGCGGTACCGCGATTTTCCTCG
TGGGTCACGTCACCAAAGAAGGCGCACTGGCCGGGCCGCGTGTTCTGGAACACATGGTCGACACCGTGTTGTATTTCGAA
GGCGAGTCAGACGGTCGTCTGCGCCTGCTGCGCGCGGTAAAGAATCGGTTTGGTGCGGTCAATGAGTTGGGCGTGTTTGG
CATGACCGACAAAGGCTTGAAAGAAGTCTCCAACCCCTCGGCAATTTTCCTGACGCGTGCCGAAGAAGAAGTGCCCGGCA
GCGTGGTGATGGCGACGTGGGAAGGCACTCGGCCGATGCTGGTGGAGGTTCAGGCGTTGGTGGATGACAGCCATTTGGCC
AATCCACGTCGGGTCACTCTCGGCCTGGACCAGAATCGTCTGGCCATGTTGCTCGCAGTCTTGCATCGCCACGGCGGTAT
TCCTACCCATGATCAGGATGTGTTCCTGAACGTGGTCGGCGGCGTGAAAGTATTGGAGACAGCTTCAGATCTGGCGCTGA
TGGCGGCTGTTATCTCCAGCCTGCGTAACCGGCCGCTGCCCCACGACCTGCTGGTGTTCGGAGAAATCGGCCTGTCAGGC
GAAGTCCGCCCGGTGCCGAGCGGTCAAGAGCGTTTGAAAGAAGCGGCCAAGCATGGCTTCAAGCGGGCTATCGTGCCCAA
AGGCAATGCACCCAAAGAAGCGCCGGCAGGGCTGCAAGTGATTGCAGTCACCCGACTTGAGCAGGCGTTGGACGCCTTGT
TCGAGTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.007

99.56

0.488
  radA Streptococcus pneumoniae Rx1

47.391

100

0.479
  radA Streptococcus pneumoniae D39

47.391

100

0.479
  radA Streptococcus pneumoniae TIGR4

47.391

100

0.479
  radA Streptococcus pneumoniae R6

47.391

100

0.479
  radA Streptococcus mitis NCTC 12261

47.174

100

0.477
  radA Streptococcus mitis SK321

47.368

100

0.475