Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ71_RS02025 Genome accession   NZ_AP027588
Coordinates   429320..429997 (-) Length   225 a.a.
NCBI ID   WP_338384439.1    Uniprot ID   -
Organism   Escherichia coli strain LR-64-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424320..434997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ71_RS02010 (VEE11_03940) chiA 425687..428380 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  AABJ71_RS02015 (VEE11_03950) bfd 428549..428743 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABJ71_RS02020 (VEE11_03960) bfr 428815..429291 (+) 477 WP_000675504.1 bacterioferritin -
  AABJ71_RS02025 (VEE11_03970) pilD 429320..429997 (-) 678 WP_338384439.1 prepilin peptidase GspO Machinery gene
  AABJ71_RS02030 (VEE11_03980) gspM 429997..430458 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABJ71_RS02035 (VEE11_03990) gspL 430455..431618 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  AABJ71_RS02040 (VEE11_04000) gspK 431633..432616 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  AABJ71_RS02045 (VEE11_04010) gspJ 432609..433196 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABJ71_RS02050 (VEE11_04020) gspI 433189..433566 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABJ71_RS02055 (VEE11_04030) gspH 433563..434072 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABJ71_RS02060 (VEE11_04040) gspG 434080..434517 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24970.85 Da        Isoelectric Point: 7.6353

>NTDB_id=87643 AABJ71_RS02025 WP_338384439.1 429320..429997(-) (pilD) [Escherichia coli strain LR-64-1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYELIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=87643 AABJ71_RS02025 WP_338384439.1 429320..429997(-) (pilD) [Escherichia coli strain LR-64-1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGAATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378