Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RE654_RS21095 Genome accession   NZ_CP133757
Coordinates   4545164..4546603 (+) Length   479 a.a.
NCBI ID   WP_041211850.1    Uniprot ID   A0A2L0TRY9
Organism   Aeromonas caviae strain LZSFT54     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4540164..4551603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE654_RS21065 (RE654_21065) mlaF 4540742..4541542 (-) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -
  RE654_RS21070 (RE654_21070) - 4541749..4542744 (+) 996 WP_202043038.1 KpsF/GutQ family sugar-phosphate isomerase -
  RE654_RS21075 (RE654_21075) kdsC 4542744..4543298 (+) 555 WP_041211851.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RE654_RS21080 (RE654_21080) lptC 4543295..4543855 (+) 561 WP_069785530.1 LPS export ABC transporter periplasmic protein LptC -
  RE654_RS21085 (RE654_21085) lptA 4543842..4544360 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  RE654_RS21090 (RE654_21090) lptB 4544373..4545098 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  RE654_RS21095 (RE654_21095) rpoN/rpoN1 4545164..4546603 (+) 1440 WP_041211850.1 RNA polymerase factor sigma-54 Machinery gene
  RE654_RS21100 (RE654_21100) hpf 4546624..4546911 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  RE654_RS21105 (RE654_21105) ptsN 4546914..4547360 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RE654_RS21110 (RE654_21110) rapZ 4547401..4548267 (+) 867 WP_010675869.1 RNase adapter RapZ -
  RE654_RS21115 (RE654_21115) npr 4548288..4548560 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  RE654_RS21120 (RE654_21120) - 4548576..4550480 (-) 1905 WP_309563192.1 HTH domain-containing protein -
  RE654_RS21125 (RE654_21125) - 4550584..4551324 (-) 741 WP_045522210.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53731.54 Da        Isoelectric Point: 4.3728

>NTDB_id=876381 RE654_RS21095 WP_041211850.1 4545164..4546603(+) (rpoN/rpoN1) [Aeromonas caviae strain LZSFT54]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQAETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=876381 RE654_RS21095 WP_041211850.1 4545164..4546603(+) (rpoN/rpoN1) [Aeromonas caviae strain LZSFT54]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGGCAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACAGCTCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAAGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCGGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TRY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482