Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   RDJ20_RS26770 Genome accession   NZ_CP133755
Coordinates   5564331..5565923 (+) Length   530 a.a.
NCBI ID   WP_196745313.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain BJ86     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5559331..5570923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDJ20_RS26750 (RDJ20_26750) pgeF 5561081..5561809 (-) 729 WP_058179501.1 peptidoglycan editing factor PgeF -
  RDJ20_RS26755 (RDJ20_26755) rluD 5561806..5562768 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  RDJ20_RS26760 (RDJ20_26760) - 5562914..5563939 (+) 1026 WP_033972916.1 outer membrane protein assembly factor BamD -
  RDJ20_RS26765 (RDJ20_26765) - 5564096..5564341 (+) 246 WP_003102591.1 PP0621 family protein -
  RDJ20_RS26770 (RDJ20_26770) pilS 5564331..5565923 (+) 1593 WP_196745313.1 two-component system sensor histidine kinase PilS Regulator
  RDJ20_RS26775 (RDJ20_26775) pilR 5565938..5567275 (+) 1338 WP_042168494.1 two-component system response regulator PilR Regulator
  RDJ20_RS26780 (RDJ20_26780) thiO 5567321..5568415 (-) 1095 WP_033972971.1 glycine oxidase ThiO -
  RDJ20_RS26785 (RDJ20_26785) - 5568550..5569059 (+) 510 WP_039028119.1 GspH/FimT family pseudopilin -
  RDJ20_RS26790 (RDJ20_26790) - 5569165..5569671 (+) 507 WP_003134935.1 GspH/FimT family pseudopilin -
  RDJ20_RS26795 (RDJ20_26795) pilV 5569662..5570219 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59100.33 Da        Isoelectric Point: 6.5650

>NTDB_id=876349 RDJ20_RS26770 WP_196745313.1 5564331..5565923(+) (pilS) [Pseudomonas aeruginosa strain BJ86]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVLPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELVAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGADDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLVRDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=876349 RDJ20_RS26770 WP_196745313.1 5564331..5565923(+) (pilS) [Pseudomonas aeruginosa strain BJ86]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
AGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAATATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCAGCGGCCAGCCTCG
GGCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAAGAACTCAACGCATTGATCCTGCAGCGTATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCACCGACGCTCAAGGTGCT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCCGAGGAGCTGGTCGCCCCGGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAT
AGCGAACTGCACCTGCAACTCGGTGCCGACGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGTGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

98.868

100

0.989