Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RGU78_RS15690 Genome accession   NZ_CP133712
Coordinates   3267656..3268456 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CRAb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3262656..3273456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGU78_RS15670 (RGU78_15695) - 3263721..3264419 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  RGU78_RS15675 (RGU78_15700) hisS 3264448..3265740 (-) 1293 WP_000095250.1 histidine--tRNA ligase -
  RGU78_RS15680 (RGU78_15705) ispG 3265737..3266852 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RGU78_RS15685 (RGU78_15710) - 3266874..3267665 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  RGU78_RS15690 (RGU78_15715) pilF 3267656..3268456 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RGU78_RS15695 (RGU78_15720) rlmN 3268472..3269704 (-) 1233 WP_002055119.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  RGU78_RS15700 (RGU78_15725) ndk 3269827..3270258 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  RGU78_RS15705 (RGU78_15730) iscX 3270372..3270572 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  RGU78_RS15710 (RGU78_15735) - 3270672..3271223 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  RGU78_RS15715 (RGU78_15740) - 3271248..3272546 (-) 1299 WP_001991191.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=876025 RGU78_RS15690 WP_004738086.1 3267656..3268456(-) (pilF) [Acinetobacter baumannii strain CRAb1]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=876025 RGU78_RS15690 WP_004738086.1 3267656..3268456(-) (pilF) [Acinetobacter baumannii strain CRAb1]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726