Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   TOL5_RS18570 Genome accession   NZ_AP024708
Coordinates   4013952..4014755 (-) Length   267 a.a.
NCBI ID   WP_004825013.1    Uniprot ID   A0A0A8TUP8
Organism   Acinetobacter sp. Tol 5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4008952..4019755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL5_RS18550 (TOL5_36620) - 4010012..4010713 (-) 702 WP_004825005.1 YfgM family protein -
  TOL5_RS18555 (TOL5_36630) hisS 4010744..4012039 (-) 1296 WP_042090326.1 histidine--tRNA ligase -
  TOL5_RS18560 (TOL5_36640) ispG 4012036..4013151 (-) 1116 WP_042090325.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  TOL5_RS18565 (TOL5_36650) - 4013173..4013961 (-) 789 WP_004825011.1 helix-turn-helix domain-containing protein -
  TOL5_RS18570 (TOL5_36660) pilF 4013952..4014755 (-) 804 WP_004825013.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  TOL5_RS18575 (TOL5_36670) rlmN 4014815..4016065 (-) 1251 WP_151780519.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  TOL5_RS18580 (TOL5_36680) ndk 4016242..4016673 (-) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  TOL5_RS18585 (TOL5_36690) iscX 4016816..4017016 (-) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  TOL5_RS18590 (TOL5_36700) - 4017190..4017741 (-) 552 WP_151780520.1 phosphatase PAP2 family protein -
  TOL5_RS18595 (TOL5_36710) - 4017772..4019058 (-) 1287 WP_151780521.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29949.02 Da        Isoelectric Point: 9.2123

>NTDB_id=87599 TOL5_RS18570 WP_004825013.1 4013952..4014755(-) (pilF) [Acinetobacter sp. Tol 5]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRSGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVSERYNDAIEQLSIAGASLGYDQRYKALENLGRVYLKVGD
TANAEKAFKNALQVNRDSIISLVELSEIFYLQQKNAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=87599 TOL5_RS18570 WP_004825013.1 4013952..4014755(-) (pilF) [Acinetobacter sp. Tol 5]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTGGTACAGGGTTGTCAAACGACAAACTCAGA
CACAATTATAAAAAAAGATCCTGAGAAAGCCGTCAAAGTTCGTACCCAGTTAGCTGCGGAGTATATTCGATCAGGCGATC
TGGATGCAGCCAAACGTGCACTTGATCAAGCCCTTGAAACAGATTCACGAGATTCAGCTGCCAATATGATGATGGGGGTT
TTACTACAACAAGAAGGTAGTAAGATCAGCATGGAAAAAGCGGATGGCTATTTTAAGCGTGCGATTTCCAGTGATTCGAA
AAATGCGCAGGCTCGTAATAATTATGGAACATATTTATTTGTTAGCGAGCGTTATAATGATGCGATTGAGCAATTGAGTA
TTGCAGGCGCATCTCTTGGATACGACCAGCGTTATAAGGCTTTAGAAAATTTGGGGCGGGTATATTTAAAAGTTGGTGAT
ACAGCAAATGCCGAAAAAGCATTCAAAAATGCCCTACAAGTCAATCGCGATTCGATCATTTCTTTGGTAGAGTTGTCTGA
GATTTTCTATTTGCAACAAAAAAATGCGCAAGCAAGTCAACTCTATGAACAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGCGCAAGAGCACTCTGGATTGGTTTACGTATTGCACGTGCCAATAATGACCAGATGGGAATGCAAGTACTGGTGAAC
CAGTTGCGGGCATTATTCCCTGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(116-173)

Tetratricopeptide repeat (TPR_16)

(44-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8TUP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648

Multiple sequence alignment