Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   RFN65_RS21070 Genome accession   NZ_CP133703
Coordinates   4081131..4082243 (-) Length   370 a.a.
NCBI ID   WP_309529932.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4076131..4087243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS21055 (RFN65_21055) gyrA 4076221..4078686 (-) 2466 WP_003244540.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -
  RFN65_RS21060 (RFN65_21060) gyrB 4078897..4080813 (-) 1917 WP_003226808.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  RFN65_RS21065 (RFN65_21065) remB 4080868..4081113 (-) 246 WP_003219266.1 extracellular matrix regulator RemB -
  RFN65_RS21070 (RFN65_21070) recF 4081131..4082243 (-) 1113 WP_309529932.1 DNA replication/repair protein RecF Machinery gene
  RFN65_RS21075 (RFN65_21075) rlbA 4082259..4082474 (-) 216 WP_003226810.1 ribosome maturation protein RlbA -
  RFN65_RS21080 (RFN65_21080) dnaN 4082605..4083741 (-) 1137 WP_003226811.1 DNA polymerase III subunit beta -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42318.37 Da        Isoelectric Point: 7.7143

>NTDB_id=875874 RFN65_RS21070 WP_309529932.1 4081131..4082243(-) (recF) [Bacillus subtilis strain CP35]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSRYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQSDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=875874 RFN65_RS21070 WP_309529932.1 4081131..4082243(-) (recF) [Bacillus subtilis strain CP35]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCTCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCGGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCCGTCTACCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAATCTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTGACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.459

100

0.995