Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RFN65_RS19170 Genome accession   NZ_CP133703
Coordinates   3725944..3726393 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain CP35     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3720944..3731393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS19150 (RFN65_19150) hxlR 3722155..3722517 (-) 363 WP_015482783.1 transcriptional activator HxlR -
  RFN65_RS19155 (RFN65_19155) hxlA 3722749..3723381 (+) 633 WP_063336040.1 3-hexulose-6-phosphate synthase -
  RFN65_RS19160 (RFN65_19160) hxlB 3723387..3723944 (+) 558 WP_063336039.1 6-phospho-3-hexuloisomerase -
  RFN65_RS19165 (RFN65_19165) tlpC 3724055..3725776 (+) 1722 WP_047182067.1 methyl-accepting chemotaxis protein TlpC -
  RFN65_RS19170 (RFN65_19170) nucA/comI 3725944..3726393 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  RFN65_RS19175 (RFN65_19175) nin/comJ 3726420..3726818 (+) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  RFN65_RS19180 (RFN65_19180) bglC 3726855..3728288 (-) 1434 WP_047182066.1 6-phospho-beta-glucosidase -
  RFN65_RS19185 (RFN65_19185) - 3728360..3729088 (-) 729 WP_021481826.1 AAA family ATPase -
  RFN65_RS19190 (RFN65_19190) - 3729107..3729448 (-) 342 WP_032724911.1 DUF3147 family protein -
  RFN65_RS19195 (RFN65_19195) - 3729436..3729858 (-) 423 WP_174616579.1 MarR family winged helix-turn-helix transcriptional regulator -
  RFN65_RS19200 (RFN65_19200) yckD 3729986..3730318 (-) 333 WP_047182065.1 YckD family protein -
  RFN65_RS19205 (RFN65_19205) yckC 3730400..3730855 (-) 456 WP_024572178.1 RDD family protein -
  RFN65_RS19210 (RFN65_19210) - 3730875..3731072 (-) 198 WP_120363256.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=875867 RFN65_RS19170 WP_014475770.1 3725944..3726393(+) (nucA/comI) [Bacillus subtilis strain CP35]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=875867 RFN65_RS19170 WP_014475770.1 3725944..3726393(+) (nucA/comI) [Bacillus subtilis strain CP35]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCTGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987