Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   RFN65_RS12135 Genome accession   NZ_CP133703
Coordinates   2390338..2391231 (-) Length   297 a.a.
NCBI ID   WP_063335350.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2385338..2396231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS12120 (RFN65_12120) xerC 2385710..2386624 (-) 915 WP_003231988.1 tyrosine recombinase XerC -
  RFN65_RS12125 (RFN65_12125) trmFO 2386692..2387999 (-) 1308 WP_063335351.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  RFN65_RS12130 (RFN65_12130) topA 2388075..2390150 (-) 2076 WP_003245599.1 type I DNA topoisomerase -
  RFN65_RS12135 (RFN65_12135) dprA 2390338..2391231 (-) 894 WP_063335350.1 DNA-processing protein DprA Machinery gene
  RFN65_RS12140 (RFN65_12140) sucD 2391292..2392194 (-) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  RFN65_RS12145 (RFN65_12145) sucC 2392223..2393380 (-) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  RFN65_RS12150 (RFN65_12150) ylqH 2393553..2393834 (-) 282 WP_063335349.1 FlhB-like flagellar biosynthesis protein -
  RFN65_RS12155 (RFN65_12155) - 2393831..2395561 (-) 1731 WP_015383652.1 hypothetical protein -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32890.14 Da        Isoelectric Point: 9.8232

>NTDB_id=875841 RFN65_RS12135 WP_063335350.1 2390338..2391231(-) (dprA) [Bacillus subtilis strain CP35]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSNQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGISEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=875841 RFN65_RS12135 WP_063335350.1 2390338..2391231(-) (dprA) [Bacillus subtilis strain CP35]
TTGGATCAGGCCGCTGCCTGCTTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAACCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGAATAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTATTTGACCCTTACGCCGGAGGGCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

98.316

100

0.983
  dprA Lactococcus lactis subsp. cremoris KW2

41.729

89.562

0.374