Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   RFN65_RS09725 Genome accession   NZ_CP133703
Coordinates   1868319..1868939 (-) Length   206 a.a.
NCBI ID   WP_004399067.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1863319..1873939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS09705 (RFN65_09705) dnaD 1863599..1864297 (+) 699 WP_004398499.1 DNA replication protein DnaD -
  RFN65_RS09710 (RFN65_09710) nth 1864316..1864975 (+) 660 WP_009967580.1 endonuclease III -
  RFN65_RS09715 (RFN65_09715) ypoC 1864972..1865466 (+) 495 WP_063335222.1 YpoC family protein -
  RFN65_RS09720 (RFN65_09720) ponA 1865541..1868297 (-) 2757 WP_063335221.1 PBP1A family penicillin-binding protein -
  RFN65_RS09725 (RFN65_09725) recU 1868319..1868939 (-) 621 WP_004399067.1 Holliday junction resolvase RecU Machinery gene
  RFN65_RS09730 (RFN65_09730) yppC 1868980..1869942 (+) 963 WP_063335220.1 DUF2515 domain-containing protein -
  RFN65_RS09735 (RFN65_09735) sspM 1869967..1870071 (-) 105 WP_003225606.1 acid-soluble spore protein SspM -
  RFN65_RS09740 (RFN65_09740) yppD 1870269..1870514 (+) 246 WP_004398488.1 YppD family protein -
  RFN65_RS09745 (RFN65_09745) yppE 1870560..1870931 (+) 372 WP_063335219.1 YppE family protein -
  RFN65_RS09750 (RFN65_09750) yppF 1870970..1871158 (-) 189 WP_003230690.1 YppF family protein -
  RFN65_RS09755 (RFN65_09755) sppG 1871346..1871723 (+) 378 WP_015251832.1 YppG family protein -
  RFN65_RS09760 (RFN65_09760) ypqA 1871744..1872163 (-) 420 WP_014477158.1 Hsp20/alpha crystallin family protein -
  RFN65_RS09765 (RFN65_09765) ptsA 1872301..1872807 (+) 507 WP_015483368.1 PTS glucose transporter subunit IIA -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 23959.09 Da        Isoelectric Point: 9.6240

>NTDB_id=875837 RFN65_RS09725 WP_004399067.1 1868319..1868939(-) (recU) [Bacillus subtilis strain CP35]
MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDRKE
KNGRKSIRKDELEETAYPISLGYAPRIDYISIIEQLYFSPSSGAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=875837 RFN65_RS09725 WP_004399067.1 1868319..1868939(-) (recU) [Bacillus subtilis strain CP35]
GTGATTCGGTATCCTAACGGAAAAACATTTCAGCCGAAACATTCGGTTTCATCCCAAAACAGTCAGAAAAGGGCCCCGTC
TTACAGTAATCGCGGAATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTTA
TACACAAAAAGCCGACACCTGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGTGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCGTCGACAACAGACTACAATGGGATTTACAAAGGGCGATATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTTCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTTTATTTTTTGGAAGCCGATAAGCTGTTTTATTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTGGAAGAAACAGCTTATCCGATTTCTCTTGGATACGCACCCAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCCATCATCTGGTGCGAAAGGTTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

100

100

1