Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RFN65_RS08210 Genome accession   NZ_CP133703
Coordinates   1601009..1601776 (+) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1596009..1606776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS08180 (RFN65_08180) pgpH 1596305..1598440 (+) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -
  RFN65_RS08185 (RFN65_08185) ybeY 1598441..1598914 (+) 474 WP_100506295.1 rRNA maturation RNase YbeY -
  RFN65_RS08190 (RFN65_08190) unpK 1598895..1599266 (+) 372 WP_015384119.1 diacylglycerol kinase family protein -
  RFN65_RS08195 (RFN65_08195) - 1599385..1599795 (+) 411 WP_003230049.1 cytidine deaminase -
  RFN65_RS08200 (RFN65_08200) era 1599776..1600681 (+) 906 WP_003230051.1 GTPase Era -
  RFN65_RS08205 (RFN65_08205) yqzL 1600829..1600972 (+) 144 WP_003230053.1 YqzL family protein -
  RFN65_RS08210 (RFN65_08210) recO 1601009..1601776 (+) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  RFN65_RS08215 (RFN65_08215) glyQ 1602074..1602961 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  RFN65_RS08220 (RFN65_08220) glyS 1602954..1604993 (+) 2040 WP_309530230.1 glycine--tRNA ligase subunit beta -
  RFN65_RS08225 (RFN65_08225) ccpN 1605126..1605764 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  RFN65_RS08230 (RFN65_08230) ppsR 1605790..1606602 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=875825 RFN65_RS08210 WP_015384118.1 1601009..1601776(+) (recO) [Bacillus subtilis strain CP35]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=875825 RFN65_RS08210 WP_015384118.1 1601009..1601776(+) (recO) [Bacillus subtilis strain CP35]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATACTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992