Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WD728_RS02565 Genome accession   NZ_CP149810
Coordinates   536842..538275 (-) Length   477 a.a.
NCBI ID   WP_021559534.1    Uniprot ID   -
Organism   Escherichia coli strain EP91B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531842..543275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WD728_RS02530 (WD728_02530) elbB 532533..533186 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  WD728_RS02535 (WD728_02535) mtgA 533183..533911 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WD728_RS02540 (WD728_02540) yrbL 533908..534540 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  WD728_RS02545 (WD728_02545) npr 534754..535026 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WD728_RS02550 (WD728_02550) rapZ 535023..535877 (-) 855 WP_000243741.1 RNase adapter RapZ -
  WD728_RS02555 (WD728_02555) ptsN 535923..536414 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WD728_RS02560 (WD728_02560) hpf 536532..536819 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WD728_RS02565 (WD728_02565) rpoN/rpoN1 536842..538275 (-) 1434 WP_021559534.1 RNA polymerase factor sigma-54 Machinery gene
  WD728_RS02570 (WD728_02570) lptB 538323..539048 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WD728_RS02575 (WD728_02575) lptA 539055..539612 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WD728_RS02580 (WD728_02580) lptC 539581..540156 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WD728_RS02585 (WD728_02585) kdsC 540153..540719 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WD728_RS02590 (WD728_02590) kdsD 540740..541726 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WD728_RS02595 (WD728_02595) yrbG 541740..542717 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53974.81 Da        Isoelectric Point: 4.3663

>NTDB_id=875717 WD728_RS02565 WP_021559534.1 536842..538275(-) (rpoN/rpoN1) [Escherichia coli strain EP91B]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYLGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=875717 WD728_RS02565 WP_021559534.1 536842..538275(-) (rpoN/rpoN1) [Escherichia coli strain EP91B]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCTGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465