Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | RFN60_RS12785 | Genome accession | NZ_CP133653 |
| Coordinates | 2492375..2492785 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain CP38 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2487741..2539234 | 2492375..2492785 | within | 0 |
Gene organization within MGE regions
Location: 2487741..2539234
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RFN60_RS12760 (RFN60_12760) | yqeF | 2487908..2488639 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| RFN60_RS12765 (RFN60_12765) | cwlH | 2488891..2489643 (-) | 753 | WP_015251672.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| RFN60_RS12770 (RFN60_12770) | yqeD | 2489830..2490456 (+) | 627 | WP_015251671.1 | TVP38/TMEM64 family protein | - |
| RFN60_RS12775 (RFN60_12775) | gnd | 2490475..2491368 (-) | 894 | WP_015251670.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| RFN60_RS12780 (RFN60_12780) | yqeB | 2491620..2492342 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| RFN60_RS12785 (RFN60_12785) | nucA/comI | 2492375..2492785 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| RFN60_RS12790 (RFN60_12790) | - | 2492981..2493331 (+) | 351 | Protein_2473 | sigma-70 family RNA polymerase sigma factor | - |
| RFN60_RS12795 (RFN60_12795) | spoIVCA | 2493359..2494819 (-) | 1461 | WP_223257626.1 | site-specific DNA recombinase SpoIVCA | - |
| RFN60_RS12800 (RFN60_12800) | - | 2494777..2494955 (-) | 179 | Protein_2475 | hypothetical protein | - |
| RFN60_RS12805 (RFN60_12805) | arsC | 2495310..2495729 (-) | 420 | WP_004398596.1 | thioredoxin-dependent arsenate reductase | - |
| RFN60_RS12810 (RFN60_12810) | acr3 | 2495741..2496781 (-) | 1041 | WP_032722149.1 | arsenite efflux transporter Acr3 | - |
| RFN60_RS12815 (RFN60_12815) | arsK | 2496804..2497244 (-) | 441 | WP_032722151.1 | ArsI/CadI family heavy metal resistance metalloenzyme | - |
| RFN60_RS12820 (RFN60_12820) | arsR | 2497303..2497620 (-) | 318 | WP_029318103.1 | arsenical resistance operon transcriptional regulator ArsR | - |
| RFN60_RS12825 (RFN60_12825) | yqcI | 2497992..2498756 (-) | 765 | WP_017696291.1 | YqcI/YcgG family protein | - |
| RFN60_RS12830 (RFN60_12830) | rapE | 2499199..2500326 (+) | 1128 | WP_004398842.1 | response regulator aspartate phosphatase RapE | - |
| RFN60_RS12835 (RFN60_12835) | phrE | 2500316..2500450 (+) | 135 | WP_004398770.1 | phosphatase RapE inhibitor PhrE | - |
| RFN60_RS12840 (RFN60_12840) | - | 2500560..2500718 (+) | 159 | WP_003245945.1 | hypothetical protein | - |
| RFN60_RS12845 (RFN60_12845) | yqcG | 2501088..2502683 (+) | 1596 | WP_004399034.1 | LXG family T7SS effector endonuclease toxin YqcG | - |
| RFN60_RS12850 (RFN60_12850) | yqcF | 2502698..2503276 (+) | 579 | WP_009967790.1 | type VII secretion system immunity protein YqcF | - |
| RFN60_RS12855 (RFN60_12855) | - | 2503394..2503540 (+) | 147 | WP_009967791.1 | hypothetical protein | - |
| RFN60_RS12860 (RFN60_12860) | - | 2503537..2503899 (-) | 363 | WP_003229947.1 | hypothetical protein | - |
| RFN60_RS12865 (RFN60_12865) | - | 2503915..2504394 (-) | 480 | WP_004399085.1 | hypothetical protein | - |
| RFN60_RS12870 (RFN60_12870) | cwlA | 2504559..2505377 (-) | 819 | WP_017697459.1 | N-acetylmuramoyl-L-alanine amidase CwlA | - |
| RFN60_RS12875 (RFN60_12875) | skhD | 2505422..2505844 (-) | 423 | WP_017697460.1 | holin family protein | - |
| RFN60_RS12880 (RFN60_12880) | xepAK | 2505890..2506783 (-) | 894 | WP_032722160.1 | hypothetical protein | - |
| RFN60_RS12885 (RFN60_12885) | yqcE | 2506871..2507035 (-) | 165 | WP_003229944.1 | XkdX family protein | - |
| RFN60_RS12890 (RFN60_12890) | yqcD | 2507032..2507367 (-) | 336 | WP_009967793.1 | XkdW family protein | - |
| RFN60_RS12895 (RFN60_12895) | yqcC | 2507377..2508477 (-) | 1101 | WP_032722161.1 | pyocin knob domain-containing protein | - |
| RFN60_RS12900 (RFN60_12900) | - | 2508481..2508753 (-) | 273 | WP_032722163.1 | hypothetical protein | - |
| RFN60_RS12905 (RFN60_12905) | yqcA | 2508750..2509328 (-) | 579 | WP_032722164.1 | YmfQ family protein | - |
| RFN60_RS12910 (RFN60_12910) | yqbT | 2509312..2510358 (-) | 1047 | WP_032722165.1 | baseplate J/gp47 family protein | - |
| RFN60_RS12915 (RFN60_12915) | yqbS | 2510351..2510776 (-) | 426 | WP_032722166.1 | DUF2634 domain-containing protein | - |
| RFN60_RS12920 (RFN60_12920) | yqbR | 2510789..2511055 (-) | 267 | WP_032722167.1 | DUF2577 family protein | - |
| RFN60_RS12925 (RFN60_12925) | yqbQ | 2511052..2512032 (-) | 981 | WP_032722168.1 | hypothetical protein | - |
| RFN60_RS12930 (RFN60_12930) | yqbP | 2512045..2512704 (-) | 660 | WP_032722169.1 | LysM peptidoglycan-binding domain-containing protein | - |
| RFN60_RS12935 (RFN60_12935) | yqbO | 2512697..2517454 (-) | 4758 | WP_043940167.1 | phage tail tape measure protein | - |
| RFN60_RS12940 (RFN60_12940) | - | 2517457..2517594 (-) | 138 | WP_021480099.1 | hypothetical protein | - |
| RFN60_RS12945 (RFN60_12945) | - | 2517636..2518085 (-) | 450 | WP_032722171.1 | phage tail assembly chaperone | - |
| RFN60_RS12950 (RFN60_12950) | txpA | 2518231..2518410 (+) | 180 | WP_004398662.1 | type I toxin-antitoxin system toxin TxpA | - |
| RFN60_RS12955 (RFN60_12955) | bsrH | 2518790..2518879 (+) | 90 | WP_075058862.1 | type I toxin-antitoxin system toxin BsrH | - |
| RFN60_RS12960 (RFN60_12960) | yqbM | 2519133..2519576 (-) | 444 | WP_003229930.1 | phage tail tube protein | - |
| RFN60_RS12965 (RFN60_12965) | yqbK | 2519579..2520979 (-) | 1401 | WP_003229929.1 | phage tail sheath family protein | - |
| RFN60_RS12970 (RFN60_12970) | - | 2520980..2521171 (-) | 192 | WP_010886574.1 | hypothetical protein | - |
| RFN60_RS12975 (RFN60_12975) | yqbJ | 2521168..2521605 (-) | 438 | WP_003229927.1 | DUF6838 family protein | - |
| RFN60_RS12980 (RFN60_12980) | yqbI | 2521618..2522121 (-) | 504 | WP_003246050.1 | HK97 gp10 family phage protein | - |
| RFN60_RS12985 (RFN60_12985) | yqbH | 2522118..2522480 (-) | 363 | WP_003229925.1 | YqbH/XkdH family protein | - |
| RFN60_RS12990 (RFN60_12990) | gkpG | 2522477..2522872 (-) | 396 | WP_004398566.1 | DUF3199 family protein | - |
| RFN60_RS12995 (RFN60_12995) | yqbF | 2522876..2523187 (-) | 312 | WP_003229923.1 | YqbF domain-containing protein | - |
| RFN60_RS13000 (RFN60_13000) | skdG | 2523198..2524133 (-) | 936 | WP_309520476.1 | phage major capsid protein | - |
| RFN60_RS13005 (RFN60_13005) | yqbD | 2524152..2525120 (-) | 969 | WP_003229921.1 | XkdF-like putative serine protease domain-containing protein | - |
| RFN60_RS13010 (RFN60_13010) | - | 2525153..2525806 (-) | 654 | WP_003229920.1 | hypothetical protein | - |
| RFN60_RS13015 (RFN60_13015) | yqbB | 2525847..2526764 (-) | 918 | WP_004398748.1 | phage head morphogenesis protein | - |
| RFN60_RS13020 (RFN60_13020) | yqbA | 2526761..2528293 (-) | 1533 | WP_004398894.1 | phage portal protein | - |
| RFN60_RS13025 (RFN60_13025) | stmB | 2528297..2529592 (-) | 1296 | WP_003229917.1 | PBSX family phage terminase large subunit | - |
| RFN60_RS13030 (RFN60_13030) | terS | 2529585..2530304 (-) | 720 | WP_003229916.1 | phage terminase small subunit | - |
| RFN60_RS13035 (RFN60_13035) | - | 2530372..2530836 (-) | 465 | WP_004398685.1 | hypothetical protein | - |
| RFN60_RS13040 (RFN60_13040) | yqaQ | 2530980..2531435 (-) | 456 | WP_004398775.1 | hypothetical protein | - |
| RFN60_RS13045 (RFN60_13045) | - | 2531633..2532562 (+) | 930 | WP_003229913.1 | hypothetical protein | - |
| RFN60_RS13050 (RFN60_13050) | yqaO | 2532636..2532842 (-) | 207 | WP_003229912.1 | XtrA/YqaO family protein | - |
| RFN60_RS13055 (RFN60_13055) | yqaN | 2532924..2533352 (-) | 429 | WP_009967809.1 | RusA family crossover junction endodeoxyribonuclease | - |
| RFN60_RS13060 (RFN60_13060) | - | 2533448..2533597 (-) | 150 | WP_003229910.1 | hypothetical protein | - |
| RFN60_RS13065 (RFN60_13065) | sknM | 2533588..2534529 (-) | 942 | WP_075058863.1 | ATP-binding protein | - |
| RFN60_RS13070 (RFN60_13070) | yqaL | 2534411..2535088 (-) | 678 | WP_116362964.1 | DnaD domain-containing protein | - |
| RFN60_RS13075 (RFN60_13075) | recT | 2535164..2536018 (-) | 855 | WP_003229907.1 | recombinase RecT | - |
| RFN60_RS13080 (RFN60_13080) | yqaJ | 2536021..2536980 (-) | 960 | WP_004398673.1 | YqaJ viral recombinase family protein | - |
| RFN60_RS13085 (RFN60_13085) | - | 2537086..2537280 (-) | 195 | WP_003229905.1 | YqaI family protein | - |
| RFN60_RS13090 (RFN60_13090) | - | 2537240..2537413 (-) | 174 | WP_119123069.1 | hypothetical protein | - |
| RFN60_RS13095 (RFN60_13095) | sknH | 2537410..2537667 (-) | 258 | WP_003245994.1 | YqaH family protein | - |
| RFN60_RS13100 (RFN60_13100) | yqaG | 2537664..2538233 (-) | 570 | WP_004398626.1 | helix-turn-helix transcriptional regulator | - |
| RFN60_RS13105 (RFN60_13105) | - | 2538307..2538447 (-) | 141 | WP_003229902.1 | hypothetical protein | - |
| RFN60_RS13110 (RFN60_13110) | yqaF | 2538477..2538707 (-) | 231 | WP_004398958.1 | helix-turn-helix transcriptional regulator | - |
| RFN60_RS13115 (RFN60_13115) | sknR | 2538884..2539234 (+) | 351 | WP_004398704.1 | transcriptional regulator SknR | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=875586 RFN60_RS12785 WP_009967785.1 2492375..2492785(-) (nucA/comI) [Bacillus subtilis strain CP38]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=875586 RFN60_RS12785 WP_009967785.1 2492375..2492785(-) (nucA/comI) [Bacillus subtilis strain CP38]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |