Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RFN60_RS12535 Genome accession   NZ_CP133653
Coordinates   2448931..2449698 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain CP38     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2443931..2454698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS12515 (RFN60_12515) ppsR 2444106..2444918 (-) 813 WP_015251688.1 pyruvate, water dikinase regulatory protein -
  RFN60_RS12520 (RFN60_12520) ccpN 2444944..2445582 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  RFN60_RS12525 (RFN60_12525) glyS 2445715..2447754 (-) 2040 WP_048655099.1 glycine--tRNA ligase subunit beta -
  RFN60_RS12530 (RFN60_12530) glyQ 2447747..2448634 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  RFN60_RS12535 (RFN60_12535) recO 2448931..2449698 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  RFN60_RS12540 (RFN60_12540) yqzL 2449735..2449878 (-) 144 WP_003230053.1 YqzL family protein -
  RFN60_RS12545 (RFN60_12545) era 2450026..2450931 (-) 906 WP_015251686.1 GTPase Era -
  RFN60_RS12550 (RFN60_12550) - 2450912..2451322 (-) 411 WP_032722130.1 cytidine deaminase -
  RFN60_RS12555 (RFN60_12555) unpK 2451441..2451812 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  RFN60_RS12560 (RFN60_12560) ybeY 2451793..2452266 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  RFN60_RS12565 (RFN60_12565) pgpH 2452267..2454402 (-) 2136 WP_015251683.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=875582 RFN60_RS12535 WP_014477369.1 2448931..2449698(-) (recO) [Bacillus subtilis strain CP38]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=875582 RFN60_RS12535 WP_014477369.1 2448931..2449698(-) (recO) [Bacillus subtilis strain CP38]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996