Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   RFN60_RS12250 Genome accession   NZ_CP133653
Coordinates   2397967..2399004 (-) Length   345 a.a.
NCBI ID   WP_044052501.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP38     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2392967..2404004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS12200 (RFN60_12200) tasA 2393064..2393849 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  RFN60_RS12205 (RFN60_12205) sipW 2393913..2394485 (-) 573 WP_003230181.1 signal peptidase I SipW -
  RFN60_RS12210 (RFN60_12210) tapA 2394469..2395230 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  RFN60_RS12215 (RFN60_12215) yqzG 2395502..2395828 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  RFN60_RS12220 (RFN60_12220) spoIITA 2395870..2396049 (-) 180 WP_029726723.1 YqzE family protein -
  RFN60_RS12225 (RFN60_12225) comGG 2396121..2396495 (-) 375 WP_015251714.1 ComG operon protein ComGG Machinery gene
  RFN60_RS12230 (RFN60_12230) comGF 2396496..2396879 (-) 384 WP_015251713.1 ComG operon protein ComGF Machinery gene
  RFN60_RS12235 (RFN60_12235) comGE 2396905..2397252 (-) 348 WP_021480020.1 ComG operon protein 5 Machinery gene
  RFN60_RS12240 (RFN60_12240) comGD 2397236..2397667 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  RFN60_RS12245 (RFN60_12245) comGC 2397657..2397953 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  RFN60_RS12250 (RFN60_12250) comGB 2397967..2399004 (-) 1038 WP_044052501.1 comG operon protein ComGB Machinery gene
  RFN60_RS12255 (RFN60_12255) comGA 2398991..2400061 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  RFN60_RS12260 (RFN60_12260) - 2400274..2400471 (-) 198 WP_014480259.1 CBS domain-containing protein -
  RFN60_RS12265 (RFN60_12265) corA 2400473..2401426 (-) 954 WP_015483432.1 magnesium transporter CorA -
  RFN60_RS12270 (RFN60_12270) yqhB 2401569..2402897 (+) 1329 WP_003230150.1 hemolysin family protein -
  RFN60_RS12275 (RFN60_12275) rsbRD 2402950..2403786 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39746.21 Da        Isoelectric Point: 9.6257

>NTDB_id=875580 RFN60_RS12250 WP_044052501.1 2397967..2399004(-) (comGB) [Bacillus subtilis strain CP38]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=875580 RFN60_RS12250 WP_044052501.1 2397967..2399004(-) (comGB) [Bacillus subtilis strain CP38]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTTGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.69

93.623

0.933