Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   RFB12_RS00380 Genome accession   NZ_CP133588
Coordinates   70361..70657 (-) Length   98 a.a.
NCBI ID   WP_015864482.1    Uniprot ID   A0A6G9J654
Organism   Parageobacillus toebii strain NEB857     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 65361..75657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB12_RS00345 (RFB12_00345) - 65925..67592 (+) 1668 WP_062752898.1 DEAD/DEAH box helicase -
  RFB12_RS00350 (RFB12_00350) - 67579..68370 (+) 792 WP_015864476.1 YqhG family protein -
  RFB12_RS00355 (RFB12_00355) - 68431..68610 (-) 180 WP_015864477.1 YqzE family protein -
  RFB12_RS00360 (RFB12_00360) comGG 68805..69191 (-) 387 WP_015864478.1 competence type IV pilus minor pilin ComGG -
  RFB12_RS00365 (RFB12_00365) comGF 69208..69630 (-) 423 WP_081188125.1 competence type IV pilus minor pilin ComGF -
  RFB12_RS00370 (RFB12_00370) comGE 69627..69950 (-) 324 WP_062678736.1 competence type IV pilus minor pilin ComGE -
  RFB12_RS00375 (RFB12_00375) comGD 69934..70377 (-) 444 WP_062678737.1 competence type IV pilus minor pilin ComGD -
  RFB12_RS00380 (RFB12_00380) comGC 70361..70657 (-) 297 WP_015864482.1 competence type IV pilus major pilin ComGC Machinery gene
  RFB12_RS00385 (RFB12_00385) comGB 70672..71697 (-) 1026 WP_309218339.1 competence type IV pilus assembly protein ComGB -
  RFB12_RS00390 (RFB12_00390) - 71790..72212 (-) 423 Protein_77 ATPase, T2SS/T4P/T4SS family -
  RFB12_RS00395 (RFB12_00395) - 72432..72917 (+) 486 WP_309218340.1 PH domain-containing protein -
  RFB12_RS00400 (RFB12_00400) - 72947..73534 (-) 588 WP_309218341.1 hypothetical protein -
  RFB12_RS00405 (RFB12_00405) - 73616..73834 (-) 219 WP_309218342.1 hypothetical protein -
  RFB12_RS00410 (RFB12_00410) - 74269..75291 (-) 1023 WP_309218343.1 hypothetical protein -

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 10889.74 Da        Isoelectric Point: 6.8588

>NTDB_id=875198 RFB12_RS00380 WP_015864482.1 70361..70657(-) (comGC) [Parageobacillus toebii strain NEB857]
MNEKGFTLIEMLIVLMVISILLLIVIPNITKHNGMINNKGCEAFVNTVQAQVKAYEMEHKQIPTIQQLIDGKYIKSDHCP
NGHKIQINSNGEVSESGA

Nucleotide


Download         Length: 297 bp        

>NTDB_id=875198 RFB12_RS00380 WP_015864482.1 70361..70657(-) (comGC) [Parageobacillus toebii strain NEB857]
ATGAATGAAAAAGGGTTTACGTTAATCGAAATGTTAATTGTATTAATGGTTATTTCTATTTTATTGCTTATTGTCATTCC
AAATATAACGAAACACAATGGAATGATTAATAACAAAGGATGTGAAGCGTTTGTGAACACCGTCCAAGCCCAAGTGAAGG
CGTATGAAATGGAACATAAACAAATTCCGACAATACAGCAGCTTATTGATGGAAAGTATATTAAGTCAGACCATTGTCCA
AACGGACATAAAATTCAAATTAATAGCAACGGGGAAGTAAGTGAAAGTGGCGCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9J654

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

57.895

96.939

0.561
  comGC Staphylococcus aureus MW2

45.161

94.898

0.429
  comGC Staphylococcus aureus N315

45.161

94.898

0.429