Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RFB14_RS25565 Genome accession   NZ_CP133587
Coordinates   5245277..5246647 (-) Length   456 a.a.
NCBI ID   WP_171506335.1    Uniprot ID   -
Organism   Brevibacillus borstelensis strain NEB264     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5240277..5251647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB14_RS25540 (RFB14_25540) ispD 5240710..5241402 (-) 693 WP_122958195.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RFB14_RS25545 (RFB14_25545) - 5241477..5242568 (-) 1092 WP_003392038.1 PIN/TRAM domain-containing protein -
  RFB14_RS25550 (RFB14_25550) - 5242860..5243255 (+) 396 WP_003392040.1 hypothetical protein -
  RFB14_RS25555 (RFB14_25555) pssA 5243347..5244075 (-) 729 WP_003392042.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  RFB14_RS25560 (RFB14_25560) disA 5244190..5245266 (-) 1077 WP_003392045.1 DNA integrity scanning diadenylate cyclase DisA -
  RFB14_RS25565 (RFB14_25565) radA 5245277..5246647 (-) 1371 WP_171506335.1 DNA repair protein RadA Machinery gene
  RFB14_RS25570 (RFB14_25570) - 5246768..5249221 (-) 2454 WP_003392048.1 ATP-dependent Clp protease ATP-binding subunit -
  RFB14_RS25575 (RFB14_25575) - 5249317..5250381 (-) 1065 WP_003392050.1 protein arginine kinase -
  RFB14_RS25580 (RFB14_25580) - 5250400..5250918 (-) 519 WP_024984559.1 UvrB/UvrC motif-containing protein -
  RFB14_RS25585 (RFB14_25585) - 5250947..5251411 (-) 465 WP_003392054.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49229.68 Da        Isoelectric Point: 6.6398

>NTDB_id=875191 RFB14_RS25565 WP_171506335.1 5245277..5246647(-) (radA) [Brevibacillus borstelensis strain NEB264]
MSKYKTKYACQECGYESPKWMGKCPGCNNWNTMVEEVAVKTASRHESLHGGQRQTAALPLTEVAGEEEPRMDTTIGELNR
VLGGGLVPGSMVLVGGDPGIGKSTLLLQTSFALAHGGIKVLYVSGEESAKQIKLRADRLGMSTPPMYVLAENDLELIEQH
IAQVDPQVLIIDSIQTVFHPAVQSAAGSVAQVRETTSQLMRIAKGKGIATFIVGHVTKEGAIAGPRMLEHMVDAVLYFEG
ERHNTFRILRAVKNRFGSTNEIGIFEMKEKGLEEVGNPSEIFLAERPVGVAGSTVVASMEGTRPVLVELQALVAPTSFVT
PRRMATGVDHQRVGMIMAVLEKRMGLMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEVGLTGE
VRGVSRIEQRIREAHKLGFKRVIIPSKNIRGLDVPSDIEVVGVSNISEALSEVLRG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=875191 RFB14_RS25565 WP_171506335.1 5245277..5246647(-) (radA) [Brevibacillus borstelensis strain NEB264]
ATGTCCAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCCAAATGGATGGGGAAATGTCCTGGATG
CAACAATTGGAATACGATGGTGGAAGAGGTTGCGGTAAAGACCGCCTCCCGCCATGAGAGTTTGCATGGCGGACAGCGGC
AAACAGCAGCCCTTCCGCTGACAGAGGTGGCCGGCGAAGAAGAGCCGCGGATGGATACAACGATAGGTGAATTAAACCGG
GTTCTGGGCGGAGGGCTCGTTCCCGGCTCAATGGTTCTGGTCGGCGGAGACCCGGGGATCGGGAAGTCTACCTTGCTGTT
GCAAACGTCGTTCGCGCTTGCACACGGGGGAATAAAGGTCTTGTACGTATCCGGCGAGGAGTCCGCCAAGCAAATCAAAT
TGCGTGCCGATCGGCTCGGAATGAGCACGCCGCCGATGTATGTGCTTGCAGAAAACGATTTGGAGCTCATTGAGCAGCAC
ATTGCCCAGGTCGATCCCCAGGTCCTGATCATCGACTCGATTCAGACTGTCTTCCATCCTGCTGTCCAATCAGCGGCGGG
GAGTGTCGCACAGGTGAGGGAAACAACCTCTCAACTGATGCGAATCGCCAAGGGAAAAGGTATCGCGACATTTATCGTCG
GTCACGTGACGAAGGAAGGCGCGATTGCCGGGCCCCGCATGCTGGAGCATATGGTGGATGCGGTCTTGTACTTTGAAGGG
GAACGGCATAATACGTTCCGCATTTTGCGAGCAGTAAAAAACCGCTTTGGCTCGACGAATGAAATCGGCATCTTTGAGAT
GAAAGAAAAGGGGCTCGAGGAAGTTGGAAACCCTTCGGAAATCTTTTTGGCAGAACGTCCGGTCGGCGTCGCTGGCTCGA
CTGTAGTGGCCAGCATGGAGGGAACCAGGCCGGTTCTGGTAGAGCTTCAGGCGCTGGTTGCACCGACCAGCTTTGTGACC
CCGCGTCGGATGGCTACCGGCGTGGATCACCAGCGAGTCGGCATGATCATGGCAGTGCTTGAGAAGCGCATGGGTCTCAT
GCTGCAAAATCAGGATGCCTATGTCAACGTGGCTGGCGGTGTTCGTTTGGATGAGCCCGCTGTCGATTTGGCGATAGCTG
TCAGCATTGCCAGCAGCTTTCGCGATCACGCGACCAATCCCCATGATGTCGTGATCGGGGAAGTGGGATTAACGGGTGAA
GTCCGCGGCGTGTCCCGGATTGAACAGCGGATTCGCGAAGCGCACAAGCTAGGATTCAAACGGGTGATCATCCCGTCCAA
AAATATCCGCGGTCTTGACGTTCCATCGGACATCGAGGTAGTCGGTGTAAGCAATATTTCGGAAGCATTAAGCGAAGTGC
TAAGGGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.376

100

0.667
  radA Streptococcus pneumoniae Rx1

58.462

99.781

0.583
  radA Streptococcus pneumoniae D39

58.462

99.781

0.583
  radA Streptococcus pneumoniae R6

58.462

99.781

0.583
  radA Streptococcus pneumoniae TIGR4

58.462

99.781

0.583
  radA Streptococcus mitis NCTC 12261

58.462

99.781

0.583
  radA Streptococcus mitis SK321

58.462

99.781

0.583