Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RE735_RS18515 Genome accession   NZ_CP133585
Coordinates   3491007..3492386 (-) Length   459 a.a.
NCBI ID   WP_309174098.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3486007..3497386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS18495 (RE735_18495) ispF 3487491..3487967 (-) 477 WP_309174095.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  RE735_RS18500 (RE735_18500) ispD 3487971..3488660 (-) 690 WP_309174096.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RE735_RS18505 (RE735_18505) - 3488674..3489774 (-) 1101 WP_309175538.1 PIN/TRAM domain-containing protein -
  RE735_RS18510 (RE735_18510) disA 3489925..3491004 (-) 1080 WP_309174097.1 DNA integrity scanning diadenylate cyclase DisA -
  RE735_RS18515 (RE735_18515) radA 3491007..3492386 (-) 1380 WP_309174098.1 DNA repair protein RadA Machinery gene
  RE735_RS18520 (RE735_18520) clpC 3492480..3494915 (-) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  RE735_RS18525 (RE735_18525) - 3494912..3496003 (-) 1092 WP_309174099.1 protein arginine kinase -
  RE735_RS18530 (RE735_18530) - 3496008..3496565 (-) 558 WP_309174100.1 UvrB/UvrC motif-containing protein -
  RE735_RS18535 (RE735_18535) - 3496580..3497044 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50011.41 Da        Isoelectric Point: 7.1218

>NTDB_id=875135 RE735_RS18515 WP_309174098.1 3491007..3492386(-) (radA) [Bacillus aerius strain NEB1378]
MAKSKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDQNQNVLYISGEESIKQTKLRADRLGIESSSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=875135 RE735_RS18515 WP_309174098.1 3491007..3492386(-) (radA) [Bacillus aerius strain NEB1378]
ATGGCTAAGTCAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCCGGCTG
CGGCACTTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCGGCAAATCGTCGAAGTGCGTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCACTTGTTCTTATTGGCGGTGATCCTGGGATTGGGAAGTCCACACT
ATTATTACAAGTTTCAGCACAGCTATCAGATCAAAATCAGAATGTGTTATACATATCTGGTGAGGAATCAATCAAACAAA
CGAAGCTTAGGGCGGACCGTCTCGGTATTGAAAGTTCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCTGCTTTTGTTGTGGTCGATTCAATTCAAACCGTTTATCAAAGCGATATCACATCAGC
TCCTGGATCTGTTTCTCAGGTGAGGGAATGCACTGCCCAGCTGATGAAAATTGCCAAGACGAATGGTATTCCTATTTTTA
TCGTCGGTCACGTGACGAAGGAAGGCTCGATTGCTGGGCCGCGCCTTTTAGAGCATATGGTAGATACAGTTCTTTATTTT
GAAGGGGAGCGCCATCATACGTTTCGTATTTTACGGGCAGTGAAAAACCGTTTTGGTTCAACGAATGAATTAGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACAGAGGTATTAAACCCATCAGAAATCTTCTTAGAAGAGCGGTCAGCAGGTGTATCTG
GATCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTAGTGGAGATACAAGCACTTATTTCACCGACGAGCTTT
GGGAATCCACGGAGAATGGCTACTGGCCTTGATCATAACCGAGTGTCGTTGCTTATGGCCGTTTTAGAAAAACGGGTAGG
ACTGCTGCTGCAAAACCAAGATGCCTATTTAAAGGTCGCAGGTGGTGTGAAACTTGATGAACCAGCGATTGACTTGGCTA
TTGCCGTTAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCAGATTGCTTTATAGGGGAAGTGGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCTAAACTAGGATTTAAACGAATGTTTATTCC
TCAGGCAAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTGGTCGGTGTAGAAAATGTAGCAGAGGCACTTCGTA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

62.031

98.693

0.612
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612
  radA Streptococcus mitis SK321

62.031

98.693

0.612