Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RE735_RS17250 Genome accession   NZ_CP133585
Coordinates   3260895..3261323 (+) Length   142 a.a.
NCBI ID   WP_309173942.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3255895..3266323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS17240 (RE735_17240) - 3256490..3258469 (-) 1980 WP_309173940.1 hydantoinase B/oxoprolinase family protein -
  RE735_RS17245 (RE735_17245) - 3258459..3260528 (-) 2070 WP_309173941.1 hydantoinase/oxoprolinase family protein -
  RE735_RS17250 (RE735_17250) nucA/comI 3260895..3261323 (+) 429 WP_309173942.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RE735_RS17255 (RE735_17255) - 3261450..3263147 (+) 1698 WP_309173943.1 methyl-accepting chemotaxis protein -
  RE735_RS17260 (RE735_17260) comJ 3263284..3263670 (+) 387 WP_309173944.1 competence protein ComJ -
  RE735_RS17265 (RE735_17265) - 3263672..3264478 (-) 807 WP_309173945.1 AAC(3) family N-acetyltransferase -
  RE735_RS17270 (RE735_17270) - 3264509..3264922 (-) 414 Protein_3375 Lrp/AsnC family transcriptional regulator -
  RE735_RS17275 (RE735_17275) - 3265023..3265787 (+) 765 WP_309173946.1 MBL fold metallo-hydrolase -
  RE735_RS17280 (RE735_17280) - 3265834..3266163 (-) 330 WP_309173947.1 YckD family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 16094.02 Da        Isoelectric Point: 5.5985

>NTDB_id=875134 RE735_RS17250 WP_309173942.1 3260895..3261323(+) (nucA/comI) [Bacillus aerius strain NEB1378]
MKLLKTILLLLLIVISVATGYVQLEQNKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKNSDEQRDKS
LHDIPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWISHQLSDDPDGTRIQFIIE

Nucleotide


Download         Length: 429 bp        

>NTDB_id=875134 RE735_RS17250 WP_309173942.1 3260895..3261323(+) (nucA/comI) [Bacillus aerius strain NEB1378]
ATGAAACTTCTTAAAACCATTCTCTTGCTTTTATTGATTGTCATTAGTGTAGCAACAGGCTATGTCCAACTAGAGCAAAA
TAAACAAGAGACGACAAACTCTTCTTATGACAAAACCATTCACTTTCCATCAGATCGATATCCAGAAACCGCGAAACACA
TAGAAGAAGCAATTGACGAAGGTCATTCTTCCATTTGCACCATTGATCGAAAAAATAGTGATGAGCAAAGAGACAAATCA
CTTCATGACATTCCAACAAAGCGTGGTTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGTGGAACAGGGGC
TTCTGTCAAATATGTACGTCCTTCGGATAATAGGGGGGCAGGCTCTTGGATAAGTCATCAATTATCAGATGATCCTGATG
GCACAAGAATCCAATTTATTATCGAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.345

83.803

0.514