Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RE735_RS08675 Genome accession   NZ_CP133585
Coordinates   1613077..1614147 (+) Length   356 a.a.
NCBI ID   WP_309175318.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1608077..1619147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS08640 (RE735_08640) - 1609159..1609473 (+) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -
  RE735_RS08645 (RE735_08645) - 1609498..1609671 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  RE735_RS08650 (RE735_08650) - 1609832..1610470 (+) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  RE735_RS08655 (RE735_08655) - 1610720..1610962 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  RE735_RS08660 (RE735_08660) - 1611174..1611554 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RE735_RS08670 (RE735_08670) - 1611780..1612652 (+) 873 WP_309175317.1 STAS domain-containing protein -
  RE735_RS08675 (RE735_08675) comGA 1613077..1614147 (+) 1071 WP_309175318.1 competence type IV pilus ATPase ComGA Machinery gene
  RE735_RS08680 (RE735_08680) comGB 1614128..1615168 (+) 1041 WP_309175319.1 competence type IV pilus assembly protein ComGB -
  RE735_RS08685 (RE735_08685) comGC 1615185..1615478 (+) 294 WP_309175320.1 competence type IV pilus major pilin ComGC Machinery gene
  RE735_RS08690 (RE735_08690) comGD 1615471..1615917 (+) 447 WP_309175321.1 competence type IV pilus minor pilin ComGD -
  RE735_RS08695 (RE735_08695) comGE 1615901..1616212 (+) 312 WP_309175322.1 competence type IV pilus minor pilin ComGE -
  RE735_RS08700 (RE735_08700) comGF 1616157..1616654 (+) 498 WP_309175323.1 competence type IV pilus minor pilin ComGF -
  RE735_RS08705 (RE735_08705) - 1616651..1617034 (+) 384 WP_309175324.1 competence type IV pilus minor pilin ComGG -
  RE735_RS08710 (RE735_08710) - 1617095..1617289 (+) 195 WP_268360764.1 YqzE family protein -
  RE735_RS08715 (RE735_08715) - 1617554..1617788 (+) 235 Protein_1695 transposase -
  RE735_RS08720 (RE735_08720) - 1617846..1618172 (-) 327 WP_309175325.1 DUF3889 domain-containing protein -
  RE735_RS08725 (RE735_08725) tapA 1618425..1618952 (+) 528 WP_309175326.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40364.69 Da        Isoelectric Point: 9.0449

>NTDB_id=875108 RE735_RS08675 WP_309175318.1 1613077..1614147(+) (comGA) [Bacillus aerius strain NEB1378]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRAGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTIVAISAQRLIELSCPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEARL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=875108 RE735_RS08675 WP_309175318.1 1613077..1614147(+) (comGA) [Bacillus aerius strain NEB1378]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGGG
AGCGGTTAATTGCTCATTTCAAATTTTTATCCTCTATGGATATTGGTGAAAGAAGGAGACCCCAGAATGGGTCATTGGCT
GTGATGCTAAGAGCCGGTCAAGTGTTTGTGCGGCTGTCTACATTACCGACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTACCGAAAACAAAATATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCGGGGAAAACGACAACACTTTATTCGTTGATCCAATTT
GCCAAAAAGCATTTTAATCGAAATATCATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCCATTAGAGCAGCTTTGACTGGTCATTTAGTCATGAGCACACTGCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTTGAATTTGGTGTGACAATGAATGAACTCGAGCAAACAATAGTTGC
CATTTCTGCTCAGCGGTTAATCGAACTTTCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACATATCA
TCGATGGGTTTATGAAGAAGCGCGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646